Effects of an Amino acid Deficiency on Hyaluronan Synthesis in Human Dermal Fibroblasts

Abstract

Molecules involved in skin function are greatly affected by nutritional conditions. However, the mechanism linking amino acid (AA)s with these alterations is not well understood. We examined the effects of an AA deficiency on hyaluronan synthesis in human dermal fibroblasts. A deficiency of all AAs or of valine, leucine, isoleucine, threonine, methionine, cysteine, phenylalanine, tyrosine, tryptophan, lysine, arginine, histidine, or glutamine significantly decreased hyaluronan levels in the culture supernatant. The deficiencies of all AAs, valine, leucine, cysteine or glutamine tended to decrease hyaluronan synthase 2 mRNA levels in human dermal fibroblasts. The present study would contribute to the elucidation of the mechanisms underlying the effects of AAs on skin function.