Optimization and Application of Ultrasound Assisted QuEChERS and Ionic Liquid Dispersive Liquid–liquid Microextraction Followed by HPLC for Determination of BBP and DBP in Packaging Food

Abstract

A simple, rapid and efficient method named ultrasound-assisted QuEChERS (quick, easy, cheap, effective, rugged, and safe) and ionic liquid dispersive liquid-liquid microextraction (IL-DLLME) prior to high performance liquid chromatography with photodiode array detection (HPLC-PAD) was developed for the determination of butyl benzyl phthalate (BBP) and di-n-butyl phthalate (DBP) from packaged fowl meat products. The optimum conditions for IL-DLLME experiments were tracked by response surface methodology (RSM). Under the optimum conditions, the precision of proposed method less than RSD 4.0% and linear working range of 5 to 500 µg L⁻¹ with R₂ above 0.9985 were achieved. The LOD was verified as 0.25 and 0.52 µg L⁻¹ for BBP and DBP, respectively. The mean recoveries ranged from 86% to 98% with RSD less than 10.1%. The proposed method was successfully detected the presence of BBP, DBP in canned and plastic food. No significant increase of migration of the two analytes from packages to food contacted during 9 months of shelf life was detected.