Effects of Deer Antler Base Polypeptide on Hormone-induced Mammary Hyperplasia in Mice

Abstract

The objective of this study is to evaluate the effects and therapeutic mechanism of deer antler base polypeptide on mammary hyperplasia in female mice. A trail was conducted on seventy mice which were divided into seven groups including the groups of control, mock, Chinese traditional medicine, and Western medicine, as well as high-dose, medium-dose, and low-dose of polypeptide from deer antler. A combination of oestrogen-progestin induced the mammary hyperplasia. We used an intraperitoneal injection in a 30 d trial and used intragastric administration to treat the disease for a further four weeks. The body weight, organ coefficient, serum hormone level and oxidation level, as well as the pathological section of breast tissue were assessed. Statistical analysis was performed using factor analysis of variance. Compared with the mock group, the high-dose group could increase the serum T-SOD, and GSH-PX activities and thymus coefficient (P < 0.01), decrease the serum MDA concentration and the coefficient of ovary and uterus (P < 0.01). In addition, the high-dose group had reduced levels of E₂, PRL, and improved P, and LH as confirmed by ELISA to regulate hormone secretion to normal (P < 0.01). The pathological section of breast tissue produced by HE staining showed that the high-dose group reduced the number of inflammatory cells, glandular secretion, the hyperplasia and dilatation of the duct epithelium, and recovered the atrophic alveolar. The results revealed that deer antler base polypeptide has positive effects in the treatment of HMG and the prevention of breast cancer.