Food Science and Technology Research
Online ISSN : 1881-3984
Print ISSN : 1344-6606
ISSN-L : 1344-6606
Original papers
Online Enrichment Combined with High Performance Liquid Chromatography for Quantitation of Trace-level Chloramphenicol in Milk
Shunyang ZhangShijie LiMing LeiZhonghui Han
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2018 Volume 24 Issue 6 Pages 963-969

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Abstract

With growing concerns over food safety, more accurate, simpler, and faster detection methods are urgently required. An online enrichment system with a molecularly imprinted polymer (MIP) for chloramphenicol (CAP) was coupled to HPLC using a flow-injection pump and developed and validated for the analysis of trace levels of CAP in milk. Compared with direct injection into the HPLC, the developed analytical method had a lower limit of detection (0.01 µg L−1) and an enrichment factor of 364 because of the specific adsorption of CAP on the MIP. The linear range of the method was 0.05 µg L−1 to 10 mg L−1 (r2 > 0.99). After online purification and enrichment, the trace amounts of CAP in milk could be quantified, with limit of detection in milk of 0.1 µg kg−1 and recoveries ranging from 82.6% to 98.1% at different spiked levels of CAP in raw milk, and commercial liquid milk.

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© 2018 by Japanese Society for Food Science and Technology
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