Preventive Effects of Lactobacillus plantarum CQPC07 on Colitis Induced by Dextran Sodium Sulfate in Mice

Abstract

In this study, the preventive effects of Lactobacillus plantarum CQPC07 (LP) on dextran sodium sulfate (DSS)-induced male C57BL/6J mice colitis were investigated. The results showed that LP can increase the colon length and decrease the ratio of colon weight/colon length in DSS-induced colitis mice. Analyses of the sera of mice showed that LP can reduce the level of serum endothelin (ET), substance P (SP), and interleukin-10 (IL-10), enhance the activity of somatostatin (SS) and vasoactive intestinal peptide (VIP), and increase the levels of IL-2. Biochemical analyses of colonic tissues from mice showed that LP can increase the levels of glutathione (GSH), superoxide dismutase (SOD), and decrease the level of myeloperoxidase (MPO), malondialdehyde (MDA). A pathological tissue evaluation showed that LP can inhibit colon tissue damage in DSS-treated mice. Furthermore, LP can also increase neuronal nitric oxide synthase (nNOS), endothelial nitric oxide synthase (eNOS), c-Kit, stem cell factor (SCF) mRNA expression and decrease inducible nitric oxide synthase (iNOS), IL-8, and C-X-C motif chemokine receptor 2 (CXCR2) mRNA levels in the colon tissues of colitis mice. Thus, LP has a colitis inhibitory effect, the higher the concentration, the better the effect, and the effect of high concentration of LP is close to the drug of sulfasalazine.