Chemical Profile, Quality and Antioxidant Properties of Palmitoleic Acid Rich Oil from Decaisnea insignis Seeds by Different Extraction Techniques

抄録

Four extraction techniques including enzyme-assisted supercritical carbon dioxide extraction (ESC-CO₂), supercritical carbon dioxide extraction (SC-CO₂), ultrasound-assisted extraction (UAE) and Soxhlet extraction (SE) were evaluated to efficiently extract the oil from Decaisnea insignis seeds in this study. Lipid profiling by GC-MS confirmed seven fatty acids and six phytosterols in all oils obtained, in which palmitoleic acid (from 475.4 ± 12.0 by SE to 604.9 ± 26.1 mg/g oil by ESC-CO₂) and β-sitosterol (from 35.6 ± 1.2 by SE to 60.9 ± 2.3 mg cholestanol equivalent/100 g oil by ESC-CO₂) predominated, respectively. Furthermore, the oil yielded by ESC-CO₂ displayed higher polyphenol content and stronger DPPH and ABTS radical scavenging capacities. These results suggested that D. insignis seed oil is considered an alternative source of palmitoleic acid, and ESC-CO₂ could be explored as an environmentally friendly technology for extracting plant oil.