Purification, characterization and anticoagulant activity in vitro of polysaccharides from the seeds of Toona sinensis (A.Juss) Roem

Abstract

The crude polysaccharides were extracted from the seeds of Toona sinensis using deionized water, and further purified with DEAE-Sepharose CL-6B column to afford STSP-1, STSP-2, STSP-3 and STSP-4. The high efficiency gel filtration chromatography (HPGFC) analysis showed that the average molecular weight of polysaccharides (STSP-2, STSP-3) were 21.4 kDa and 43.7 kDa, separately. Monosaccharide analysis detected arabinose, rhamnose, glucosamine, galactose, glucose, xylose, mannose, glucuronic acid and galacturonic acid. Moreover, their in vitro anticoagulant activities were evaluated by activated partial thromboplastin time (APTT), thrombin time (TT) and prothrombin time (PT) assays. In comparison with the control group(saline), STSP-3 could prolong PT and TT significantly, but not APTT. STSP-3 exhibited potent anticoagulant activity and would be explored as a natural potential anticoagulant.