Analysis of the Promoter Activity of the Taka-Amylase Gene and the Phosphoglycerate Kinase Gene in a Shoyu-koji Mold Aspergillus oryzae KBN616

Abstract

Promoter activity of the genes encoding Taka-amylase A and phosphoglycerate kinase of Aspergillus oryzae was analyzed using Escherichia coli β-glucuronidase as a reporter in a shoyu-koji mold A. oryzae KBN616. Assay of the β-glucuronidase extracted from the mycelia of the transformants grown in the media containing different carbon sources suggests that expression of the Taka-amylase A gene was not induced by starch in A. oryzae KBN616. Analysis of proteins in the culture supernatant of A. oryzae KBN616 after cultivation in starch medium supports this result. The phosphoglycerate kinase gene of A. oryzae KBN616 was shown to be expressed constitutively in the medium containing glucose and starch.