Abstract
I have studied the enzymatic syntheses of oligosaccharides composing the sugar chains of glycoconjugates using glycosidases by two types of reactions: reverse hydrolysis and transglycosylation. In the reverse hydrolysis reaction, oligosaccharides having a variety of linkages were obtained by a batch method. On the other hand, it has been clarified that glycosidase having a different substrate specificity and from a different origin gives a different oligosaccharide with respective regioselectivity by the column method. In the transglycosylation reaction, the oligosaccharide was synthesized regioselectively according to the substrate specificity of the glycosidase used.
