Journal of Health Science
Online ISSN : 1347-5207
Print ISSN : 1344-9702
ISSN-L : 1344-9702
RESEARCH LETTERS
Identification of Oil-Soluble Coal Tar Dyes in Cosmetics Using Reversed-Phase TLC/Scanning Densitometry
Tsutomu OhnoEiichi MikamiHiroshi Matsumoto
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2003 年 49 巻 5 号 p. 401-404

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A simple and rapid analytical method of oil-soluble coal tar dyes in cosmetics was established using reversed-phase TLC/scanning densitometry. Eleven kinds of oil-soluble coal tar dyes were able to be separated completely on reversed-phase TLC plates by the complementary use of 2 solvent systems. The solvent systems were A; n-hexane/2-butanone solution (5 : 1, v/v), solvent system B; acetonitrile/methanol solution (5 : 1, v/v). Then we measured visible absorption spectra of spots developed on the reversed-phase TLC plates by scanning densitometer to identify these coal tar dyes. The proposed method was successfully applied to the identification of oil-soluble coal tar dyes in commercial cosmetics.
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© 2003 by The Pharmaceutical Society of Japan
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