Journal of Health Science
Online ISSN : 1347-5207
Print ISSN : 1344-9702
ISSN-L : 1344-9702
The Effect of Generation 2 and 3 Poly(amidoamine) Dendrimers on Viability of Human Breast Cancer Cells
Katarzyna WinnickaKrzysztof BielawskiMagorzata RusakAnna Bielawska
ジャーナル フリー

2009 年 55 巻 2 号 p. 169-177


In this study, we investigated the effect of amine- and hydroxyl-terminated Generation 2 and 3 (G2 and G3) poly(amidoamine) (PAMAM) dendrimers on the viability and induction of apoptosis in human MCF-7 and MDA-MB-231 breast cancer cells. It was shown that amino-terminated PAMAM dendrimers were cytotoxic, with IC50 value after 24 hr of incubation in MCF-7 and MDA-MB-231 breast cancer cells 153±3 μM and 140±2 μM for G2 PAMAM and 120±3 μM and 99±2 μM for G3 PAMAM, respectively. Experiments made with annexin V-fluorescein isothiocyanate (V-FITC) and detection of apoptosis by a fluorescent microscopy assay revealed that G2 and G3 PAMAM-NH2 dendrimers inhibited the proliferation of MCF-7 and MDA-MB-231 malignant cells by increasing the number of apoptotic and necrotic cells. The cytotoxic and apoptotic effect of G2 and G3 PAMAM-OH dendrimers was significantly weaker.

© 2009 by The Pharmaceutical Society of Japan
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