ジクロルフルオレッセンによるブロムワレリル尿素の吸光度定量法
1968 年 14 巻 2 号 p. 72-75
詳細
1968 年 14 巻 2 号 p. 72-75
A new method for the spectrophotometric determination of bromvalerylurea was established upon the reaction of 2', 7'-dichlorofluorescein and bromine. The method improved the usual method with fluorescein in the points of sensitivity of reaction and the linearity of calibration curve. Bromvalerylurea is extracted with ether from a sample containing 20-100μg of it. After the removal of ether, the extract is heated with 1 ml of ammonia water for 20 min on a water bath at 100° and evaporated. The dried residue was warmed for 20 min on a water bath at 40° after the addition with 2 drops of hydrogen peroxide and 1 ml of 2', 7'-dichlorofluorescein solution in glacial acetic acid (250 mg/liter). The reacted solution is added with 3 ml of pyridine and determined spectrophotometrically at 530 mμ. Bromdiethylacetylurea is determined similarly. The determination of bromvalerylurea in pharmaceutical preparations was carried out by this method and good results were obtained. Further study is required to apply this method for biological materials.
