Quantitative Detection of Metallothionein by High Performance Liquid Chromatography-Atomic Absorption Spectrophotometry

抄録

Metals in metallothionein and related metal-binding proteins in the soluble fraction of animal tissues were quantitatively determined by high performance liquid chromatography-atomic absorption spectrophotometry using ethylenediaminetetraacetic acid-metal complexes as internal standards. It was shown that peak areas rather than peak heights of metals have to be plotted versus internal standards and that the column has to be washed well with chelating agents to remove metals adsorbed on the column before application of internal standards.