Sep-Pak C₁₈カートリッジによるパラコートの選択的抽出

抄録

Sep-Pak C₁₈ cartridge was applied to selective extraction of paraquat from water, human urine, human blood, cow's milk and beverages. 1) Paraquat in distilled water was quantitatively retained on Sep-Pak C₁₈ cartridge by adjusting the sample to pH 13, and quantitatively recovered by elution of 2 ml of 0.1_N hydrochloric acid from the cartridge. 2) A sample (1-5ml) adjusted pH to 13 with 1_N sodium hydroxide solution was injected into a Sep-Pak C₁₈ cartridge, 3 ml of distilled water put into the cartridge to eliminate other components, and then 2 ml of 0.1_N hydrochloric acid and 3×1 ml of distilled water were passed through the cartridge to elute paraquat. Prior to application to the cartridge, a blood sample was deproteinized with perchloric acid. The eluate was filled up to 5 ml with distilled water for determination of paraquat. No interferance was observed for colorimetric determination after reduction of paraquat in alkaline sodium dithionite solution. 3) Paraquat in spiked materials was quantitatively detected at the level of 10μg, and the minimum detectable amount of the herbicide was more or less 2μg with this method. As a pretreatment for analysis of drugs in biological samples by instrumental analyses such as gas chromatography, mass spectrometry and high performance liquid chromatography, Sep-Pak C₁₈ is very useful for its cleanliness, simplicity, rapidity, efficiency and reproducibility.