高速液体クロマトグラフィーによる清涼飲料に混入されたカルバリルおよびプロパニルの定量

抄録

Carbaryl and propanil contained in soft drink were determined simulataneously by high performance liquid chromatography with a UV detector by use of stainless column (4.0mm×0.15m) packed with Nucleosil ₅C₁₈. Mobile phase was methanol-H₂O (60 : 40). The sample solution adjusted to pH 12 with ammonia after adding α-amylase solution was extracted with ether by using a column of Extrelut. Determination was carried out successfully by using clotiazepam as an internal standard and by maintaining the temperature at 50°C. Each recovery of carbaryl and propanil from soft drink (NAC : 30μg/0.2ml), (DCPA : 80μg/0.2ml) was 97.6±2.9% and 99.5±2.3%. Each content of carbaryl and propanil detected in the sample (1.248g) was 0.208 mg and 1.436 mg.