抄録
Amongst other examples of modern technology, mass spectrometry (MS) has made extraordinary progress in the last decade in terms of sensitivity, precision and the possible range of mass measurement, progress which could not be foreseen ten years ago. Starting in 1970, a method after the method of ionization was rapidly developed in tandem with dramatic improvements and innovations in MS equipment, as a result of which the range of applications of mass spectrometry has expanded exponentially, allowing MS to assume very important new roles in various fields of the life sciences, including medicine, pharmacy and environmental chemistry. One of the most outstanding developments in MS in recent years is the ability to measure non-volatile substances such as protein, nucleic acid, glycolipids, polysaccharide, and high polarity macromolecules. The most important contribution in this development come mostly from fast atom bombardment (FAB) ionization, the latest state-of-the-art ionization methods such as electrospray (ES) ionization or ionspray (IS) ionization, new range of mass spectrometric methodology, tandem mass spectrometry (MS/MS) and time of flight mass spectrometry (TOF-MS). This review centers on an outline of the mass spectrometry of non-volatile, high polarity macromolecules, concentrating on FAB, ES, IS, MS/MS and TOF-MS.
