抄録
Extensive research has been carried out on the effect of poly unsaturated fatty acid (PUFA) on cardio-vascular disease. However, most such studies indicate the quantities of fatty acids in foodstuffs and serum in terms of area percentage. Few reports indicate absolute quantities. The quantitative determination of fatty acid area percentage has been carried out thoroughly while this has yet to be done for their absolute quantities. Also research up to the present is insufficient for clearifying the continuous analytical process as a whole. The present study for determining absolute quantities of fatty acids in serum-containing PUFA using tricosanoic acid as the internal standard, indicated it necessary to extract the total lipids in serum using the Folch procedure without adding butylated hydroxytoluene.
