TLC-酵素法による部分加水分解リン脂質中のリゾホスファチジルコリン及びホスファチジルコリンの定量
1992 年 41 巻 10 号 p. 1049-1054
詳細
1992 年 41 巻 10 号 p. 1049-1054
We describe a new method for determination of phosphatidylcholine and lysophosphatidylcholine in partial hydrolyzed phospholipids. The method consists of fractionation of cholinecontaining phospholipid by preparative TLC and enzymatic determination based on the cholineoxidase-phenol method. The method affords better specificity and shorter time than the conventional two-dimensional TLC method are possible. Reproducibility (CV<3 %) and good agreement with those of two-dimensional TLC method were confirmed. This method is thus applicable to the determination of lysophosphatidylcholine and phosphatidylcholine in partially hydrolyzed phospholipids.
