Icosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) both containing fish oil, are of interest for their physiological effects on health. Sardine oil was selectively hydrolyzed by Candida cylindracea lipase in a bioreactor equipped with a cotton-cloth disperser, and a DHA rich oil was obtained.
DHA in acyl glycerol was concentrated 2.5 times at a conversion of 72 % in a 15-stage reactor.