Z-Chromosome Specific Primers for Chicken-Quail Hybrid Blastoderm

Soichi Takagi; Tamao Ono; Akira Tsukada; Yusuke Atsumi; Shusei Mizushima; Noboru Saito; Kiyoshi Shimada

doi:10.2141/jpsa.44.209

Physiology, Reproduction and Immunology: Research Note

Z-Chromosome Specific Primers for Chicken-Quail Hybrid Blastoderm

Soichi Takagi, Tamao Ono, Akira Tsukada, Yusuke Atsumi, Shusei Mizushima, Noboru Saito, Kiyoshi Shimada

著者情報

キーワード: chicken, PCR, PKCI, quail, Z-chromosome

ジャーナルフリー

2007 年 44 巻 2 号 p. 209-212

DOI https://doi.org/10.2141/jpsa.44.209

詳細

抄録

A polymerase chain reaction (PCR) primer set for a chicken pkci gene on the Z-chromosome, chPKCI, amplified a 329 bp fragment of genomic DNA in chickens but not quail. Likewise, a PCR primer set for the quail pkci gene on the Z-chromosome, quPKCI, amplified a 95 bp fragment of genomic DNA in quail but not chickens. These chicken and quail primers validly identified chicken-quail hybrids derived from Z-chromosome carrying sperm of chicken and the hybrid derived from Z-chromosome carrying oocyte of quail, respectively.

責任著者(Corresponding author)

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