Identification and Characterization of a novel mitochondrial histone-like protein in Physarum polycephalum

Narie Sasaki

doi:10.5685/plmorphol.16.61

真正粘菌における新規ミトコンドリアヒストン様タンパク質Glomの解析

佐々木成江

著者情報

ジャーナルフリー

2004 年 16 巻 1 号 p. 61-70

DOI https://doi.org/10.5685/plmorphol.16.61

詳細

発行日: 2004 年受付日: - J-STAGE公開日: 2010/06/28 受理日: - 早期公開日: - 改訂日: -
訂正情報
訂正日: 2010/06/28 訂正理由: - 訂正箇所: PDFファイル訂正内容: -

訂正日: 2010/06/28 訂正理由: - 訂正箇所: キーワード情報訂正内容: Wrong : Key Words: Glom, mitochondrial nucleoid, histone-like protein, HMG-box
Right : Glom, mitochondrial nucleoid, histone-like protein, HMG-box

訂正日: 2010/06/28 訂正理由: - 訂正箇所: 引用文献情報訂正内容: Wrong : E
E
E
E
E
E
E
E
E
E
E
E
E
E
E
E
E
E
E
E
E
E
E
E
E
E
E
E
E
E

Right : Alevizopoulos, A., Dusserre Y., Tsai-Pflugfelder, M., von der Weid, T., Wahli, W. and Mermod, N.(1995)A proline-rich TGF-beta-responsive transcriptional activator interacts with histone H3. Genes Dev. 15: 3051-3066.
Andersson, S. G., Zomorodipour, A., Andersson, J. O., Sicheritz-Ponten, T, Alsmark, U. C., Podowski, R. M., Naslund, A. K., Eriksson, A. S., Winkler, H. H. and Kurland, C. G.(1998)The genome sequence of Rickettsia Prowazekii and the origin of mitochondria. Nature 396: 133-140.
Antoshechkin I. and Bogenhagen, D. F.(1995)Distinct roles for two purified factors in transcription of Xenopus mitochondrial DNA. Mol. Cell. Biol. 15: 7032-7042.
Barry, III, C. E., Hayes, S. F. and Hackstadt, T.(1992)Nucleoid condensation in Escherichia coli that express a chlamydial histone homolog. Science 256: 377-379.
Barry, III, C. E., Brickman, T. J. and Hackstadt, T.(1993)Hcl-mediated effects on DNA structure: a potential regulator of chlamydial development. Mol. Microbiol. 9: 273-283.
Caron, F., Jacq, C. and Rouvière-Yaniv, J.(1979)Characterization of a histone-like proteinextracted from yeast mitochondria. Proc. Natl. Acad. Sci. USA. 76: 4265-4269.
Chiang, C. M. and Roeder, R. G.(1995)Cloning of an intrinsic human TFIID subunit that interacts with multiple transcriptional activators. Science 267: 531-536.
Diffley, J. F. X. and Stillman, B.(1991). A close relative of the nuclear, chrornosomal high-mobility group protein HMG1 in yeast mitochondria. Proc. Natl. Acad. Sci. USA. 88: 7864-7868.
Fisher, R. P., Topper, J. N. and Clayton, D. A.(1987)Promoter selection in human mitochondria involves binding of a transcription factor to orientation-independent upstream regulatory elements. Cell 50: 247-258.
Gerster, T., Balmaceda, C.-G. and Roeder, R. G.(1990)The cell type-specific octamer transcription factor OTF-2 has two domains required for the activation of transcription. EMBO J. 9: 1635-1643.
Hackstadt, T., Baehr, W. and Ying, Y.(1991)Chlamydia trachomatis developmentally regulated protein is homologous to eukaryotic histone H1. Proc. Natl. Acad. Sci. USA. 88: 3937-3941.
Hoshino, T., McKenzie, T., Schmidt, S., Tanaka, T., Sueoka, N.(1987)Nucleotide sequence of Bacillus subtilis dnaB: a gene essential for DNA replication initiation and membrane attachment. Proc Natl. Acad. Sci. USA. 84: 653-657
Hines J. C. and Ray, D. S.(1998)The Crithidia fasciculata KAP1 gene encodes a highly basic protein associated with kinetoplast DNA. Mol. Biol. Parasitol. 94: 41-52.
Kawano, S., and Kuroiwa, T,(1985)Isolation and characterization of a membrane-DNA complex in the mitochondrial of Physarum polycephalum. Exp. Cell Res. 16: 460-472
Kim, T. K. and Roeder R. G.(1994)Proline-rich activator CTF1 targets the TFIIB assembly step during transcriptional activation. Proc. Natl. Acad. Sci. USA. 91: 4170-4174.
Kobayashi, T., Takahara, M., Miyagishima, S., Kuroiwa, H., Sasaki, N., Ohta, N., Mastuzaki, M. and Kuroiwa, T.(2002)Detection and localization of chloroplast-encoded HU-like protein that organizes chloroplast nucleoids. Plant Cell 14: 1579-1589.
Kuroiwa, T. and Kuroiwa, H.(1980)Inhibition of Physarum mitochondrial division by cytochalasin B. Experientia 36: 193-194.
Kuroiwa, T.(1982)Mitochondrial nuclei. Int. Rev. Cytl. 75: 1-59.
Kuroiwa, T., Ohta, T., Kuroiwa, H. and Kawano, S.(1994)Molecular and cellular mechanisms of mitochondrial nuclear division and mitochondriokinesis. Microsc. Res. Tech. 27: 220-232.
Megraw, T. L. and Chae, C. B.(1993)Functional complementarity between the HMG1-like yeast mitochondrial histone HM and the bacterial histone-like protein HU. J. Biol. Chem. 268: 12758-12763.
Mermod, N., O'Neill, E. A., Kelly T. J. and Tjian, R.(1989)The proline-rich transcriptional activator of CTF/NF-1 is distinct from the replication and DNA binding domain. Cell 58: 741-753.
Parisi, M. A. and Clayton, D. A.(1991)Similarity of human mitochondrial transcription factor l to high mobility group proteins. Science 252: 965-969.
Rouvière-Yaniv, J. and Gros, F.(1975)Characterization of a novel low-molecular-weight DNA-binding protein from Escherichia coli. Proc. Natl. Acad. Sci. USA. 72: 3428-3432.
Rouvière-Yaniv, J., Yaniv, M., and Germond, J. E.(1979). E. coli DNA binding protein HU forms nucleosome like structure with circular double-stranded DNA. Cell 17: 265-274.
Sasaki, N., Kuroiwa, H., Nishitani, C., Takano, H., Higashiyama, T. Kobayashi, T., Shirai, Y., Sakai, A., Kawano, S., Murakami-Murofushi, K., and Kuroiwa, T.(2003)Glom is a novel mitochondrial DNA packaging protein in Physarum Polyceohalum and causes intense chromatin condensation without suppressing DNA functions. Mol. Biol. Cell 14: 4758-4769 .
Takano, H., Abe, T., Sakurai, R, Moriyama, Y., Miyazawa, Y., Nozaki, H., Kawano, S., Sasaki, N. and Kuroiwa, T.(2001)The complete DNA sequence of the mitochondrial genome of Physarum polycephalum. Mol. Gen. Genet. 264: 539-545.
Tanaka, M., Clouston, W. M. and Herr W.(1994)The Oct-2 glutamine-rich and proline-rich activation domains can synergize with each other or duplicates of themselves to activate transcription. Mol. Cell. Biol. 14: 6046-6055.
Xu, C. W., Hines, J. C., Engel, M. L., Russell, D. G. and Ray, D. S.(1996)Nucleus-encoded histone H1-like proteins are associated with kinetoplast DNA in the Trypanosomatid Crithidia fasciculata. Mol. cell. Biol. 16: 564-576..
Wada, M., Kano, Y., Ogawa, T., Okazaki, T. and Imamoto F.(1988)Construction and characterization of the deletion mutant of hupA and genes in Escherichia coli. J. Mol. Biol. 204: 581-91.
Yung, B. Y. and Kornberg, A.(1988)Membrane attachment activates dnaA protein, the initiation protein of chromosome replication in Escherichia coli. Proc. Natul. Acad. Sci. USA 85: 7202-7205

抄録

要旨:生体内においてミトコンドリアDNAはタンパク質によって高度に折りたたまれたミトコンドリア核様体(ミトコンドリア核)で存在する。我々は、非常に凝縮した大型ミトコンドリア核様体を持つ生物の一つである真正粘菌Physarum polycephalumから、新規ミトコンドリアヒストン様タンパク質Glomを同定した。Glomは、N末端半分にポリプロリンを含むリジンに富む領域を持ち、C末端半分にHMG boxを持つ塩基性タンパク質であった。Glomは、ミトコンドリアDNA全体に結合し、DNAの凝縮を促進するが、その凝縮にはリジンリッチ領域が重要であった。また、Glomによる激しいDNAの凝縮は複製や転写などの機能発現を全く阻害せず、その機能発現にはポリプロリンが重要であった。さらに、Glomは大腸菌のヒストン様タンパク質であるHUの機能を相補し、その機能相補にはHMGボックス領域が重要であった。

責任著者(Corresponding author)

訂正情報

J-STAGEへの登録はこちら（無料）