抄録
If the shark muscle was preserved from putrefaction while it was yet fresh, no ammonia has been produced from the muscle, but, when the muscle was antisepticized after committing itself to growth of micro-organisms, the development of ammonia was observed. And the longer the period before its antisepticization, the more the amount of ammonia grew. It is obvious, therefore, that the development of ammonia may be proportional to the degree of the growth of micro-organisms. If the muscle was antisepticized when the amount of ammonia reached a certain point, no more production of ammonia has been observed, probably because the secretion of urease from micro-organisms was discontinued. In fact, in examining the action of urease preparation extracted from the muscle at intervals in the course of its storing time, we found that the action of urease from meat, in which the amount of ammonia was on the active increase, was very strong, but it was very weak in the lag period and in the later period of its storage.