Biochemical Studies on the Liquefaction of Fish Body-III
On Effectiveness of Fish-Autolysate to the Culture of Bacteria
1956 年 21 巻 12 号 p. 1226-1232
詳細
1956 年 21 巻 12 号 p. 1226-1232
1) A preparation which is effective in culturing bacteria instead of LIEBIG extract and peptone has been obtained by autolyzing whole fish body.
2) Experiments have shown that it is superior to the usual media when judged from the magnitude of colonies on the plate media and the turbidity of the liquid media
3) The liquid media gave a distinct indol reaction on culturing either E. coli, Staphyl. aureus or Bac. proteus. The liquid media gave the easily perceptible cholera rose reaction after a 6-hour incubation at 37°C. after innoculating Vibrio cholerae in a quantity as minute as 1/100, 000 a loopful bacteria.
4) Corynebacterium diphtheriae was able to grow on a simple two per cent solution of the present preparation with a growth rate two to three times greater than on MARTIN or LOFFLER'S media. The volutin of the bacteria grown on the preparation could be identified without fail on staining. The minimum skin reaction doss of the toxin formed was 0.000001cc.
5) It has been shown that the activity of promoting the growth of bacteria which was displayed by the preparation was in variance with the progress of the autolysis.
