血合筋肉の化学的研究-VII 血合ミオグロビン及び血液ヘモグロビンの電気泳動的性質

松浦 文雄; 橋本 周久

doi:10.2331/suisan.23.222

血合筋肉の化学的研究-VII

血合ミオグロビン及び血液ヘモグロビンの電気泳動的性質

松浦文雄, 橋本周久

著者情報

ジャーナルフリー

1957 年 23 巻 4 号 p. 222-226

DOI https://doi.org/10.2331/suisan.23.222

詳細

発行日: 1957/08/25 受付日: - J-STAGE公開日: 2008/02/29 受理日: 1957/06/11 早期公開日: - 改訂日: -
訂正情報
訂正日: 2008/02/29 訂正理由: - 訂正箇所: 論文抄録訂正内容: Wrong : Metmyoglobin (MMb), prepared from the red muscle of tuna, Parathunnus sibi, after SCHMID⁶⁾, was analyzed electrophoretically by means of the T_ISELIUS apparatus and it was found that this preparation was not homogeneous except for around the isoelectric point (Fig. 1): in addition to the main component a minute one was discerned, the mobility of which differed from that of tuna methemoglobin (MHb) at any pH (Table 1) and was removed unsuccessfully by repeating recrystallization. Horse heart MMb, tested for reference, behaved in the same way at pH 6.0 (Fig. 2 and Table 1). It is uncertain at present whether this minute or small component is the secondary product from Mb or not.
Similarly in cases of MHb's of tuna and horse, were observed plural components, the relative amounts of which were, however, fairly close in contrast with the cases of MMb's.
The electrophoretic mobility of tuna MMb is very small at least in the pH region covered in this experiment: e.g., in the phosphate buffer (containing 0.05 M N_aCl, ?? /2 0.15, pH 7.3) the mobility of tuna MMb is about one half of horse's, one seventh of carp's⁸), and one fifth of turtle's⁹).
The isoelectric point of tuna MMb was observed at pH 6.8, which agrees with H_UYS, finding¹¹⁾ while that of tuna MHb at pH 5.8 (Fig. 3).
Right : Metmyoglobin (MMb), prepared from the red muscle of tuna, Parathunnus sibi, after SCHMID⁶⁾, was analyzed electrophoretically by means of the T_ISELIUS apparatus and it was found that this preparation was not homogeneous except for around the isoelectric point (Fig. 1): in addition to the main component a minute one was discerned, the mobility of which differed from that of tuna methemoglobin (MHb) at any pH (Table 1) and was removed unsuccessfully by repeating recrystallization. Horse heart MMb, tested for reference, behaved in the same way at pH 6.0 (Fig. 2 and Table 1). It is uncertain at present whether this minute or small component is the secondary product from Mb or not.
Similarly in cases of MHb's of tuna and horse, were observed plural components, the relative amounts of which were, however, fairly close in contrast with the cases of MMb's.
The electrophoretic mobility of tuna MMb is very small at least in the pH region covered in this experiment: e.g., in the phosphate buffer (containing 0.05 M N_aCl, ?? /2 0.15, pH 7.3) the mobility of tuna MMb is about one half of horse's, one seventh of carp's⁸⁾, and one fifth of turtle's⁹⁾.
The isoelectric point of tuna MMb was observed at pH 6.8, which agrees with H_UYS, finding¹¹⁾ while that of tuna MHb at pH 5.8 (Fig. 3).

訂正日: 2008/02/29 訂正理由: - 訂正箇所: 引用文献情報訂正内容: 訂正前 : 1) 松浦文雄,橋本周久:本誌, 20, 946 (1955).
2) 松浦文雄,橋本周久:本誌, 22, 408 (1956).
3) 松浦文雄,橋本周久:昭和30年10月28日日本水産学会大会(長崎)講演.
4) HAUROWITZ, F. & HARDIN, R. L.: “The Proteins”. Academic Press, New York (1954), Vol. II, Part A, p. 324.
5) 松浦文雄,橋本周久:未発表.
7) 例えばPAULING, L.: Sci. 110, 543 (1949).
16) 松浦文雄,橋本周久:昭和32年4月3日日本水産学会年会(東京)講演.

訂正後 : 1) 松浦文雄,橋本周久:本誌, 20, 946 (1955).
2) 松浦文雄,橋本周久:本誌, 22, 408 (1956).
3) 松浦文雄,橋本周久:昭和30年10月28日日本水産学会大会(長崎)講演.
5) 松浦文雄,橋本周久:未発表.
7) 例えばPAULING, L.: Sci. 110, 543 (1949).
16) 松浦文雄,橋本周久:昭和32年4月3日日本水産学会年会(東京)講演.

訂正日: 2008/02/29 訂正理由: - 訂正箇所: PDFファイル訂正内容: -

抄録

Metmyoglobin (MMb), prepared from the red muscle of tuna, Parathunnus sibi, after SCHMID⁶⁾, was analyzed electrophoretically by means of the T_ISELIUS apparatus and it was found that this preparation was not homogeneous except for around the isoelectric point (Fig. 1): in addition to the main component a minute one was discerned, the mobility of which differed from that of tuna methemoglobin (MHb) at any pH (Table 1) and was removed unsuccessfully by repeating recrystallization. Horse heart MMb, tested for reference, behaved in the same way at pH 6.0 (Fig. 2 and Table 1). It is uncertain at present whether this minute or small component is the secondary product from Mb or not.
Similarly in cases of MHb's of tuna and horse, were observed plural components, the relative amounts of which were, however, fairly close in contrast with the cases of MMb's.
The electrophoretic mobility of tuna MMb is very small at least in the pH region covered in this experiment: e.g., in the phosphate buffer (containing 0.05 M N_aCl, ?? /2 0.15, pH 7.3) the mobility of tuna MMb is about one half of horse's, one seventh of carp's⁸⁾, and one fifth of turtle's⁹⁾.
The isoelectric point of tuna MMb was observed at pH 6.8, which agrees with H_UYS, finding¹¹⁾ while that of tuna MHb at pH 5.8 (Fig. 3).

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