日本水産学会誌
Online ISSN : 1349-998X
Print ISSN : 0021-5392
ISSN-L : 0021-5392
Glycylglycine Dipeptidaseに対する金属イオンの影響-IX
幽門垂酵素液中に於けるMgの形態
斗ケ沢 宜久
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ジャーナル フリー

1958 年 24 巻 3 号 p. 186-190

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For celite filtrate of aqueous extract of pyloric caeca turbidity producing powers of a few kinds of organic solvents were measured together with protein-N content, GG-dipeptidase activity, and Mg content. The results were as follows:
1. Volume of the precipitate formed by adding a definite sort of organic solvent decreased with decreasing final concentration for all the solvents here used. As to the precipitation velocity observed by the naked eye the following order appeared to hold: acetone > dioxane > n-propanol > ethanol > methanol.
2. Protein-N contents of the eluates separated from the precipitates formed by solvent addition under controlling the final concentration so as to become 60% and 80% increased corresponding to the rises of pH value. But any relation was not recognized of its prevalence between GG-dipeptidase activity and volume of the protein deposit.
3. As for Mg as measured by the chelate titration two different forms were found to be present, in bonito pyloric caeca, one measurable directly in the enzyme solution, the other only with ash of the solution. The former may certainly be a form of ion or salt and the latter a compound made up with protein or other substances as counterpart.
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