血合筋肉の化学的研究-VIII マグロ血合筋肉よりの結晶チトクロームCに就いて

松浦 文雄; 橋本 周久

doi:10.2331/suisan.24.216

血合筋肉の化学的研究-VIII

マグロ血合筋肉よりの結晶チトクロームCに就いて

松浦文雄, 橋本周久

著者情報

ジャーナルフリー

1958 年 24 巻 3 号 p. 216-220

DOI https://doi.org/10.2331/suisan.24.216

詳細

発行日: 1958/07/25 受付日: - J-STAGE公開日: 2008/02/29 受理日: 1958/01/23 早期公開日: - 改訂日: -
訂正情報
訂正日: 2008/02/29 訂正理由: - 訂正箇所: 論文抄録訂正内容: Wrong : Crystalline cytochrome c (Cyt) was prepared from the red muscle of tuna, Neothunnus macropterus, by the method of HAGIHARA^{4, 9}) with some modification (Fig. 1). The twicerecrystallized preparation was then analyzed for the iron content, spectral and electrophoretic properties.
The iron content of this preparation was found to be 0.487%, which is slightly higher than the values of crystalline Cyt's from other sources (0.43-0.47%)^{2, 3, 6}). The spectral characters agreed fairly well with those for amorphous Cyt's reported before¹⁾, except the lower absorption coefficient for minimum (about 535mμ), which makes the ratios of ε550/ε535 and ε520/ε535 higher (Fig. 2 and Tables 1 and 2).
The electrophoretic experiments revealed the general resemblance of pH-mobility curve of tuna Cyt to those of horse, beef, pig, and chicken¹⁸⁾, though Cyt of the former moved more rapidly than the latter in the strongly acidic or alkaline pH range (Fig. 3). The isoelectric point of tuna Cyt was found to be pH 10.2, which is slightly lower in pH value, as compared with those of all the other species above-mentioned (pH 10.7-10.9)¹⁸⁾.
Right : Crystalline cytochrome c (Cyt) was prepared from the red muscle of tuna, Neothunnus macropterus, by the method of HAGIHARA^{4, 9)} with some modification (Fig. 1). The twicerecrystallized preparation was then analyzed for the iron content, spectral and electrophoretic properties.
The iron content of this preparation was found to be 0.487%, which is slightly higher than the values of crystalline Cyt's from other sources (0.43-0.47%)^{2, 3, 6)}. The spectral characters agreed fairly well with those for amorphous Cyt's reported before¹⁾, except the lower absorption coefficient for minimum (about 535mμ), which makes the ratios of ε550/ε535 and ε520/ε535 higher (Fig. 2 and Tables 1 and 2).
The electrophoretic experiments revealed the general resemblance of pH-mobility curve of tuna Cyt to those of horse, beef, pig, and chicken¹⁸⁾, though Cyt of the former moved more rapidly than the latter in the strongly acidic or alkaline pH range (Fig. 3). The isoelectric point of tuna Cyt was found to be pH 10.2, which is slightly lower in pH value, as compared with those of all the other species above-mentioned (pH 10.7-10.9)¹⁸⁾.

訂正日: 2008/02/29 訂正理由: - 訂正箇所: PDFファイル訂正内容: -

抄録

Crystalline cytochrome c (Cyt) was prepared from the red muscle of tuna, Neothunnus macropterus, by the method of HAGIHARA^{4, 9)} with some modification (Fig. 1). The twicerecrystallized preparation was then analyzed for the iron content, spectral and electrophoretic properties.
The iron content of this preparation was found to be 0.487%, which is slightly higher than the values of crystalline Cyt's from other sources (0.43-0.47%)^{2, 3, 6)}. The spectral characters agreed fairly well with those for amorphous Cyt's reported before¹⁾, except the lower absorption coefficient for minimum (about 535mμ), which makes the ratios of ε550/ε535 and ε520/ε535 higher (Fig. 2 and Tables 1 and 2).
The electrophoretic experiments revealed the general resemblance of pH-mobility curve of tuna Cyt to those of horse, beef, pig, and chicken¹⁸⁾, though Cyt of the former moved more rapidly than the latter in the strongly acidic or alkaline pH range (Fig. 3). The isoelectric point of tuna Cyt was found to be pH 10.2, which is slightly lower in pH value, as compared with those of all the other species above-mentioned (pH 10.7-10.9)¹⁸⁾.

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