抄録
In the previous paper, remarkable changes in amount of free arginine and octopine in Sepioteuthis lessoniana FERUSSAC muscle during storage at 5°-7°C, were explained by the rapid conversion of arginine to octopine in the muscle.
In order to obtain the detailed pattern of chemical changes occuring in nitrogenous extractives in the muscle during storage, components in extractives were determined including free amino acids, ammonia, trimethylammonium compounds, nucleotides and the related compounds. The results obtained were shown in Figs. 1-4.
Chemical changes observed during the first one day were, in addition to the conversion of arginine to octopine previously reported, the degradation of adenosine monophosphate to inosine and hypoxanthine and the formation of ammonia which may be caused by the deamination of adenosine monophosphate. In the period from the first day to the third, no remarkable change was observed in amount of nitrogenous extractives. Thereafter, the degradations of trimethylamine oxide, glycine and proline occured and were followed by the formation of ammonia and trimethylamine.
The nitrogenous extractives thus determined constituted 97-99% of the total extractive nitrogen of the muscle, as shown in Fig. 5.