リノレン酸メチル自動酸化生成物のゲルクロマト法による分画とその変色力
1975 年 41 巻 1 号 p. 59-64
詳細
1975 年 41 巻 1 号 p. 59-64
Components of autoxidized methyl linolenate were fractionated on a Bio-Beads S-X3 column with benzene as the eluting solvent. The elution sequence was in the order of decreasing molecular weight: tetramer ?? trimer, dimer and monomer containing degradation products. This was confirmed by comparing their elution behaviors with those of authentic compounds, and by molecular weight analysis.
Discoloration potentialities of the eluted fractions were investigated by the treatment with nitrogenous compounds. In the case of the reaction with VBN prepared from Jack mackerel (Trachurus japonicus), or ammonia, only lipophilic discolored substances were produced, whereas in the case with non-VBN or amino acids, lipophilic and hydrophilic discolored substances were produced. Appapent differences in the discoloration potentiality-curves of lipophilic and hydrophilic discolored substances and the presence of degradation products which formed hydrophilic discolored substances by the reaction with non-VBN or amino acids but not with VBN or ammonia indicated that each discolored substance was produced by a different mechanism.
