親油性変色物質中への窒素化合物の取り込み
1975 年 41 巻 1 号 p. 65-71
詳細
1975 年 41 巻 1 号 p. 65-71
The role of nitrogenous compounds in the discoloration of autoxidized products of methyl linolenate and some characteristics of the purified discolored substances were studied.
Dimer, polymer and monomer fractions prepared from the autoxidized products by gel permeation chromatography were discolored with lipid free VBN and non-VBN from Jack mackerel (Trachrus japonicus). During discoloration, polymerization proceeded in the compounds.
Discolored substances, obtained from the dimer fraction and purified by gel permeation chromatography and solvent precipitation method, were analyzed by elementary analysis, infrared spectroscopy, paper chromatography and paper electrophoresis. Non-VBN-discolored substances contained 4.51%, nitrogen and possessed imine linkage. The nitrogenous moiety moved together with discolored spots on the paper. From the results, it was concluded that the nitrogenous compounds were incorporated into the discolored substances. On the other hand, VBN-discolored substances contained only 0.92% nitrogen, but further evidence supporting incorporation was not discerned. A possible explanation for this discrepancy in nitrogen content would reside in difference in the binding and elimination of nitrogenous compounds.
