抄録
The production and characteristics of carrageenan decomposing enzyme were studied for the cell free medium of a marine Cytophaga 1k-C783.
Artificial sea water (ASW) components were necessary for enzyme production as well as bacterial growth. But glucose, galactose and cellobiose showed inhibitory effect for bacterial growth and enzyme production.
Optimal activity was found at pH 7 and a temperature range from 25 to 50°C. However, above 50°C enzyme activity became unstable and it was totally inactivated by heat treatment at 55°C for 10min. It was also found that HgCl2, AgNO3 (1mM) and NaCl (1.5% to above) completely inhibited the enzyme activity.
