アルコール発酵母液を用いた微生物フロックによるシオミズツボワムシBrachionus plicatilisの培養

抄録

The culturing of rotifer Brachionus plicatilis using a microbial flock (AFS-Flock), as food, produced from the alcohol fermentation slop (AFS) was examined. with the aim of utilizing AFS. The AFS-Flock was found to be an effective food for rotifer. When the rotifer was fed with the AFS-Flock, the density of rotifer in the culture broth was maintained at over 200 individuals (ind.)/ml for about one month. Several strains of bacteria and yeasts were isolated from the AFS-Flock. Most isolated bacteria could not grow abundantly on the medium containing AFS as the sole source of carbon and energy. However, most of the isolated yeasts assimilated AFS rapidly and abundantly. The results of culturing rotifer using cultural cells and broth of the isolated yeasts cultured with AFS, indicated that those of the isolated yeasts were an effective food aslo. When the culture broth of most effective isolated yeast cultured with AFS was fed to the rotifer, the highest density of rotifer was at 394ind. /ml in eight days of culture time. It was concluded that the AFS-Flock is hopeful as new food for rotifer.