抄録
Studies on morphological characterization of eel leucocytes and phagocytosis against Edward-siella tarda were performed. For morphology, peroxidase-Giemsa stain was used, For in vitro phagocytosis test, both whole blood culture and separated leucocyte culture were used. The morphological study revealed: lymphocyte-like cell of 5-10μm, round, round nuclei and dark gray cytoplasm. Thrombocyte-like cell of 8-10μm in length, 3-5μm in width, spindle shape, oval nuclei and light pink cytoplasm. Neutrophil-like cell of 10-15μm, round or oval, oval or bilobed nuclei and whitish-gray or whitish cytoplasm. Monocyte-like cell of 10-15μm, round, round or oval nuclei and light to dark gray cytoplasm. Only a few neutrophil-like cells showed peroxidase positive granules. Many neutrophil-like cells and other leucocytes showed no pero-xidase positive granules. Phagocytosis was observed with thrombocyte-like, neutrophil-like and monocyte-like cells, Lymphocyte-like cells showed no phagocytosis. Thrombocyte-like cells showed very weak phagocytosis. Neutrophil-like and monocyte-like cells showed active phago-cytosis.
As results it may be appropriate to conclude that the eel leucocytes could be classified as lymphocytes, thrombocytes, neutrophils and monocytes.
