抄録
The presence of ATP-sulphurylase in 12 species of marine macroalgae was investigated using the molybdolysis assay. ATP-sulphurylase was purified from the red alga Porphyra yezoensis, which showed the highest enzyme activity among 12 species, by(NH4)2SO4 fraction, column chro-matography on Sephadex G200, Red-Sepharose CL 6B and DEAE-Sephadex A50. The purified enzyme was homogeneous with polyacrylamide gel electrophoresis. Molecular weight analysis on high-speed gel filtration and SDS-polyacrylamide gel electrophoresis suggest the enzyme is a monomeric one having a molecular weight of 53000.
