チフス菌の発育素に関する研究 (第2報)

トリプトファンの発育素としての作用機序 (其の1)

抄録

As a result of the observations of the action mechanism of tryptophane against growth of the Shikata strain of typhoid bacillus from the following 2 points, the importance of indole nucleus was determined.
1) In order to determine whether the physical metabolic products of tryptophane manifested a secondary growth promoting action during the growth of bacilli, experiments were conducted with kynurenine and kynurenic, anthranilic, hydroxyanthranilic and nicotinic acids but all were found to be ineffective.
2) Since the above-mentioned compounds are those in which the indole nucleus of tryptophane had been destroyed or secondarily cyclized, assumption was made that the reaction centre of tryptophane lay in the indole nucleus. Upon this assumption, experiments were made with 10 derivatives of indole and it was found that those derivatives in which the α-position was vacant and a specific substituent was present in the β-positon, or those in which both positions were vacant, alone possessed growth promoting effect. Experiments conducted with 17 derivatives of indolizine and pyrrol, chemical properties of which are similar to those of indole, showed that 3 derivatives of pyrrol possessed weak effect. Indole was found to be approximately equally effective as tryptophane, being 10^-7 to 10^-8mol./l, and those strains which were susceptible to tryptophane, regardless of their antigenic properties, possessed about an equal amount of susceptibility to indole.