チフス菌の発育素に関する研究 (第3報)

インドールの発育素としての作用機序

抄録

1) From the fact that indole possesses about an equal amount of growth promoting action as tryptophane against a certain group of typhoid bacilli that is susceptible to tryptophane, an observation was made regarding interrelationship between the two compounds inside the bacterial body. It was determined that a substance giving positive tryptophane-like coloration reaction was formed inside the body of bacteria cultivated in a medium containing indole.
2) Since the presence of cystine is necessary for the appearance of growth promoting effect in indole, experiments were conducted with various compounds possessing -SH and all were found effective. Sodium thiosulfate and sodium sulfide were found to possess especial cooperative effect against growth promoting action of indole, they being able to substitute for cystine. By the use of these salts, composition of the medium for typhoid bacillus growth can be simplified and it became possible to obtain facilities for the analyses of metabolic mechanism of this bacillus.