The ouabain-sensitive (Na⁺+K⁺) -ATPase activities of membrane fractions from hind-leg muscle and brain of normal and genetically dystrophic mice (C57BL/6J-dy strain) were studied, and the following results were obtained. 1) The ouabain-sensitive (Na⁺+K⁺) -ATPase activity of frozen muscle sarcolemmal fraction from normal mice was several times higher than that of fresh one. 2) The ouabain-sensitive (Na⁺+K⁺) -ATPase activity of frozen muscle sarcolemmal fraction from dystrophic mice was almost equal to that from normal one. But the muscle membrane yield from dystrophic mice was considerably low compared with the yield from normal one. 3) With brain membrane fractions, no differences were observed between normal and dystrophic mice in the ouabain-sensitive (Na⁺+K⁺) -ATPase activity as well as in the yield of membrane fractions.

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Bordetella bronchisepticawas examined on the respiratory tract of mice, rats, Mongolian gerbils, golden hamsters, guinea pigs, rabbits, household dogs and cats and cynomolgus monkeys imported from Asian countries. The organisms were detected in rats, guinea pigs, rabbits, dogs, cats and monkeys, but not in mice, Mongolian gerbils and golden hamsters. In rats, the organisms were isolated from 7 of 25 breeding colonies, and isolation rates ranged from 10.0 to 61.3% by colonies. In guinea pigs, 22 of 60 breeding colonies were positive, showing 6.7 to 58.2% in isolation rates. Two groups of rabbits were examined, one was collected from many non-closed colonies of small size and another was obtained from 3 closed colonies of large size consisting of more than 100 rabbits. Isolation rates were 3.0% in the former and 66.8% in the latter. The organisms were also detected in 5.30 of dogs and 3.20 of cats, respectively. Isolation rate of the organisms in the trachea of 48 healthy monkeys was 6.3%, but in 39 monkeys with pneumonic lesions, 16 (41.0%) harbored the organisms in their lungs.

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A practical procedure for hypophysectomy of the mouse by parapharyngeal approach has been developed by modifying the technics of Motizuki [5] and Lostroh and Jordan [4] . Postoperative care in C3H/He strain of female mice was also studied.The main points improved are as follows: (a) The dental drill bar made of a needle with 2mm outer diameter was devised for making a hole in the cranium (Fig. 2) . The bar does not grind the cranium, but cuts it in the circle, resulting in speeding up of the operation and in little bleeding, since the hole is very small. (b) Special attention was paid in breathing of mice during the operation. A longitudinal midline incision of about lcm was made in muscle encircled the trachea and a pinhole was made by sewing needle into the trachea. A small piece of cotton wool was put on the pinhole and covered by the muscle. It admirably keeps the tracheal hole open for breathing during operation (Fig. 1) . (c) After the operation, the mouse was placed in a chamber filled with an atmosphere of 95% O₂-5%CO₂gas until recovered, otherwise, all mice died in a few days after operation with inflamation of the lung. The temperature of the recovery chamber is recommended to be kept at about 28°C for ensuring the resuscitation. The intraperitoneal injections of hydrocortone-acetate (0.25mg/0.1ml) just before and 2 days after operation further ameriolated the survival rate.

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Filtration efficiency of 3 types of air filter frame and cylinder (Fig. 1) was examined by culture of the air obtained from the operating isolators at intervals of 3 to 21 days. The air of isolator was bubled in 30 ml of nutrient broth for 30 or 120 minutes (4-15 1/mm.) and each of 1 ml of the broth was inoculated into thioglycollate medium (TGC), cooked meat medium (CMM), heart infusion broth (HIB) and glucose peptone water (GPW) . The media were incubated for 7 to 14 days at 30 or 25°C. As seen in Table 1, microbes could be effectively removed through all types of filter for over 10 months, though there were a very few positive cases suggesting technically accidental contamination at sampling.

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