Seven strains of Mycobacterium lepraemurium (Douglas, Fukuoka-1, Keishicho, Kurume-42, Odessa and Osaka-No. 1 strains) were isolated on the 1% Ogawa yolk medium. These colonies were white yellow, very slow growing and rough at the isolation, but were changed gradually to smooth colony by the successive cultivation. Kurume-42 and Odessa strains were strongly rough type, especially Kurume-42 strain did not change to smooth type untill the 17th generation and became extinct on account of failure of cultivation at the 18th generation. Kurume-42 strain differed from the other 7 strains of murine leprosy bacillus on the characteristic of rough type. All strains secreted coproporphyrine on the medium and coloured to red the medium surface. Many negative tubes were seen every time in the primary isolation culture and some seeded colonies did not grow in the succesive cultivation. All strains are difficult to culture.
INH or rifampicin resistant strain was isolated on the 1% Ogawa yolk medium from murine leproma formed with INH or rifampicin resistant Hawaiian strain. Degree of the INH resistant is 16μg/ml and that of rifampicin resistant is 7μg/ml.
Seven strains and. INH or rifampicin resistant strain of Hawaiian were cultivated successively for several generations, thereafter these strains were inoculated to mouse. These strains made murine leproma in the injection site. Many globi were observed on the smear preparation of these murine lepromata.

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This is a report of a sociological study on 240 inpatients hospitalized in JALMA Leprosy Centre, India. This survey was carried out during the time ranging from October 20, 1971 to January 10, 1976. Results of the survey on the medical aspects of these patients are summarized as follows:
1. Onsets of the disease were most frequently noticed by the patients at the ages between 11 and 15 (39.1%).
2. 75% of the patients came to the hospital because of patches on their body surface. However, many of them did not know the nature of the disease until the medical staffs explained it to them.
There were considerable number of patients defaulted from the regular treatment(46.2%). Their reasons for the drop-out were the absence of pain and the frustration for the retarded improvement of the clinical symptomes of leprosy. Many of them changed to other treatment centres in expectation for the better treatment.
3. 45% of inpatients had leprosy patients in their families or relatives. Cases with leprosy parents were the most frequent (43.1%).
4. Most of the patients changed more or less their food habits after the onset of the disease. 59.7% of them stopped taking animal foods such as meat, fish and egg after they had leprosy.

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