Susceptibility to subcutaneous inoculation with M. lepraemurium, strain Hawaiian-B, of 4 mouse strains (CBA/J, CBA/N, DBA and C3H/Bi) was examined, concerning the relation between the clinical features of subcutaneous leproma at the inoculation site and the evolution of visceral lesions. Hawaiian-B strain has been maintained only by passages from C57BL/6 to C57BL/6 mice in our laboratory, since 1956. Mice of C3H, C3H/He, BALB/c, KK, DDD and C57BL/6 strains were also examined by the same manner above mentioned, as controls.
Subcutaneous lesion developed a large and soft, typically malignant type, leproma at the inoculation site in CBA/J, CBA/N, DBA and C3H/Bi mice and extensive involvement was observed in their viscera, as observed in C3H mice. Taking the characteristics of above strains of mice into our consideration, CBA/J strain mice are much more suitable to use in mouse leprosy experiments as a representative of the typically malignant.

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Two hundreds and eighty one patients receiving sulfones (DDS, Protogen or Promin) or sulfamethoxypyridazine were examined by a Dichlorophenol indophenol (DPIP) test for Glucose-6-phosphate dehydrogenase (G6PD).
The blood samples of 230 cases decolorized the dye within 80 minutes, while the residual 51 samples required 80 minutes or more. A quantitative UV test for G6PD were performed on 48 cases of these 51 patients.
The result of the UV test showed that all of patients had G6PD values between 120 and 240 mU per 109 red blood cells except one male patient (114.4 mU). His red blood cells count and the hemoglobin index were within normal range and Heinz body in the red blood cells was negative though he was receiving 50 mg of DDS everyday.

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Four hundreds and eighty-four lepromatous leprosy patients (male 399 and female 85) were registered from whole Ghatampur tahsil between 1966 and 1971 at Ghatempur clinic of JALMA, Kanpur district, U. P. state, India.
Out of them, 58 %, 279 patients (male 246 and female 33) were treated for the period of over 25 % of the scheduled period. Here, some analysis was reported about those279 patients from the view of treatment period and the effect of treatment.
Seventy percent out of 246 male lepromatous patients had turned to negative in slit skin smear examination until the data was collected in 1972-1973, and about female patients the value was below 50 %. Further, about 85 % of male patients were treated for the period of over 50 % of the scheduled period for the treatment, and the number of those patients was corresponded to 46 % of the total male lepromatous leprosy patients living in the Ghatampur tahsil at that time. The value was lower than those reported from the clinics and hospitals of other parts of India.
Better effect by the treatment was obtained in the younger patients and from the result it may be said that 'early diagnosis, early treatment' will be emphasized according to the age of the patients, too.
When patients showed higher percentages of the treatment period in the scheduled period, they could obtain the negative results of slit skin smear examination more frequently. By the result, the treatment (DDS 25-50 mg per day for the maintenance doses in the ordinary cases) seemed effective for fairly large number of lepromatous patients. And, further, it may be said that, when a patient was treated for over 50% of the scheduled period, he could have a fairly regular treatment.
A large number of patients showed negative results in the successive two time slit skin smear examination between 4th and 6th year of the scheduled period. From these results it may be concluded that when male lepromatous leprosy patients can get fairly regular treatment for 6-7 years, about 60-70 % of them will show the negative results in the successive two time slit skin smear examination.
When one showed a highly positive result in the slit skin smear examination at starting of treatment, he needed a long period for the treatment, but in some cases, when even lower positive results of it was obtained, much longer treatment period was needed for them to get the negative results of it. Some patients (not many) showed still positive results of it after taking regular treatment for over 7 years.

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BCG has been vaccinated for leprosy contact children (under 14 j) from 1963 to 1976 in Okinawa, in order to prevent leprosy.
The total number of BCG vaccinated children was 951, who were examined after 3, 6, 8, 10 and 13 years to follow-up onset of leprosy. Ten contact children among 534 have developed leprosy by 6 years-examination and its incidence rate was 18.7 _??_. No leprosy cases have been detected by after 3, 8, 10 and 13 years' examination. Theincidence rate by contact tracing examination for BCG unvaccinated contact children in same period and at same plase was 20.6‰.
No special difference was observed in leprosy incidence rate between two groups. The incidence rate of BCG trial in Okinawa was higher than other trials in foreign countries, because lepromatous ratio in Okinawa field was higher than other places and index cases with whom children contacted were mainly lepromatous type. oreoverchildren had contacted with lepromatous index cases before they were vaccinated with BCG and they already might be infected with leprosy bacilli.
So it seems that no noticeable effect was observed in BCG trial in Okinawa.

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In the previous paper, it was reported that the growth of M. lepraemurium was significantly stimulated by soluble starch. In the present paper, effects of compounds related to soluble starch on the growth of M. lepraemurium were investigated. The results obtained indicate that dextran remarkably stimulated the growth of M. lepraemurium more than soluble starch. Optimal concentration of dextran was 0.2%. Dextran having molecular weights (MW) 100, 000-200, 000 was better than those of MW. 60, 000 and over 200, 000. α-, β-and γ-cyclodextrin had the same effect as dextran. Dextrin and maltose were not effective.

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[in Japanese]

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Cultivated murine leprosy bacilli from rough colonies MHO-R_??_ and smooth colony variant (HO-S) were subcutaneously inoculated into mice of inbred strains (C3H, C57BL/6, BALB/c and DDD). Growth patterns of the bacilli of each strain and cellular reactions of host in subcutaneous tissue at the inoculation site were examined by means of the spread tissue preparation technique.
There were no remarkable differences in cellular reactions at the inoculation site among these inbred strains of mice infected with HO-R bacilli 2 days and 1 week after the infection. An acute inflammatory reaction with accumulation of many polymorphonuclears was observed at 2 days. At one week, it was disappeared and monocytes and macrophages were dominant. A vigorous granulomatous reaction consisted of polymorphonucleares and lymphocytes developed in C57BL/6, BALB/c and DDD mice at 3 weeks. In the course of the following weeks the cellular reaction was augmented in C57BL/6 and BALB/c mice and further local spread of the infection was arrested. On the other hand, in DDD mice the diminution of cellular reaction occured at 8 weeks and bacilli increased in number. In contrast, no signs of host reactions were observed in C3H mice and many murine leprosy cells were observed at the inoculation site.
C57BL/6 and BALB/c mice infected with HO-S bacilli showed essentially the same host reaction as those observed in the experiment with HO-R bacilli. The diminution of cellular reaction observed in the DDD mice infected with HO-R bacilli could not be found in those infected with HO-S bacilli and the multiplication of the bacilli was not seen. In C3H mice infected with HO-S bacilli no specific host reaction was found untill 5 weeks, but cellular reactions happened at 7 to 8 weeks which were never observed in C3H mice infected with HO-R or H-M bacilli. The bacilli decreased in number from the 8th to the 10th week but bacillary multiplication was gradually recovered at 15 weeks. The multiplication of HO-S bacilli was very slow even in C3H mice throughout the observation period.
In this study, we found that slower development of mouse leprosy with HO-S bacilli in C3H mice is due to their poor ability to multiply in vivo and the inexplicable host reaction.
The mouse strain differences in response to cultivated M. lepraemurium were also discussed on the bases of cell-mediated immunity in the host.

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