Splenic lymphocytes were collected from BCG-vaccinated ddY mice and cultured with or without the antigen. Thus obtained supernatants were tested by tissue culture techniques. Normal mouse peritoneal macrophages were infected with M. lepraemurium in vitro and the effects of MycolF (mycobacterial growth inhibiting factor) in these supernatants on the growth of the bacilli were examined. The multiplications of the bacilli were suppressed in the media added with culture supernatants of splenic lymphocytes from immunized animals, especially the one cultured with antigen for 48hr.

View full abstract

Metabolism of RFP and of the representative metabolites was individually compared by tracer method.
1. The results were discussed especially concerning the metabolites remaining for long-term iu vivo.
2. A new derivative of RFP named as ADP was synthesized. Through the similar reaction between RFP or DARFP and aromatic or alicyclic carboxylic acid chloride the pre-sumable position in RFP skeleton of conjugation such as glucuronide conjugation was discussed.
3. In vivo inhibitory effect of protein on the oxidation of RFP series to their respective quinone-forms was examined using cysteine as a model of in vivo SH residue.
4. The predominant adsorption of RFP series on IgM of calf serum was concluded.
5. The low efficacy of RFPQ and ADP on the growth of leprosy bacilli was concluded by mouse footpad method. The uselessness of DARFP was also presumed.
6. The antibacteroidal effects of RFP was compared with those of some antileprosy drugs and the influence of RFP on in vivo enterobacteria was mentioned.
7. The relation between these findings and RFP therapy was discussed.

View full abstract