It has been pointed out that it is important to provide education and childcare at accredited kindergarten centers based on the differences between the children as per the time spent by them in the institution. Therefore, we examined the motor ability and movement acquisition status of type 1 certification child and type 2 certification child in a kindergarten and childcare school cooperation type accredited kindergarten. Subjects were 129 pairs of preschoolers and their guardians enrolled in three kindergarten and childcare school cooperation type accredited kindergartens in O and K City, O Prefecture in Japan. For the analysis, 102 pairs of data were used, all of which had no missing data. All measurements and surveys were conducted in February 2021. The motor ability for children was measured in 25m running, standing long jump, and tennis ball throwing. Movement acquisition status was surveyed through a self-reported questionnaire to collect responses from the parents with two choices of “possible/impossible” for each of the 15 items related to balance, mobility, and operability. Based on the above measurement data, the motor ability and movement acquisition status among children using educational and childcare facilities were compared in this study. The findings revealed that there was no significant difference in the motor ability (25m run, standing long jump, and tennis ball throw) compared to the previous studies. In addition, it was confirmed that the acquisition rate of movements was high in the order of balance, mobility, and operability. While there was no difference in the motor ability by use form, it was confirmed that the movement acquisition rate of type 2 certification child was higher than that of those type 1 certification child. Thus, it can be concluded that the amount of life experience may affect the movement acquisition centered on operability.