Journal of Pet Animal Nutrition
Online ISSN : 2185-7601
Print ISSN : 1344-3763
ISSN-L : 1344-3763
Volume 3, Issue 2
Displaying 1-7 of 7 articles from this issue
  • The Primary Study for Measurement of Canine Body Fat Mass by Deuterium Oxide Dilution Method
    M. Sagawa, S. Yoneda, F. Nakadomo
    2000 Volume 3 Issue 2 Pages 67-71
    Published: October 01, 2000
    Released on J-STAGE: September 24, 2012
    JOURNAL FREE ACCESS
    Obesity is becoming popular in companion dogs, and for its diagnosis and therapy, assessment of adiposity is essential. Deuterium oxide (D2O) dilution method is established as one of the most reliable methods to estimate body fat content in humans, and this method was also applied to dogs in anesthesia (Burkholder et al.,1998). The purpose of this study is to determine the precondition for repeated measurement of body fat mass by the D2O dilution method, in conscious dogs. Three conscious and unrestrained beagles were intravenously injected with D2 Oat 0.2 ml/kg BW, and then their plasma were corrected at 15,30,60,90,120 and 180 minutes after the injection. Plasma samples were purified by vacuum sublimation, and their deuterium concentrations were measured by gas chromatographic method. The concentration of plasma deuterium had already reached a plateau level at 15 minutes and was not significantly changed until 180 minutes. It suggests that in conscious dogs, only 15 min is adequate time for D2O equilibration, to correct plasma in body fat measurement by D2O dilution method. Their body fat contents obtained by the method were 18,23 and 39%, which adequated their Body Condition Score (BCS),3,3 and 4, respectively. The plasma deuterium concentration was further measured at 3,7,14,28,49 and 70 days after the injection. It decreased to 36 and 74% of the plateau level at Day 3 and 7, respectively, and returned to pre-injection level at Day 49. These results indicate that the turnover rate of body water is about 4 days in dogs, and that at least a 7-week interval is required for the repeated measurement by D2O dilution method.
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  • S. OHSHIMA, Y. FUKUMA, T. SUZUKI, M. ABE
    2000 Volume 3 Issue 2 Pages 72-81
    Published: October 01, 2000
    Released on J-STAGE: September 24, 2012
    JOURNAL FREE ACCESS
    Four mature Beagles weighing 15 kg on average were used to determine digestible energy (DE) and digestible crude protein (DCP) contents of 20 samples of 17 feedstuffs, and nitrogencorrected metabolizable energy (MEn) was individually estimated with an equation [MEn = -DE1.25DCP], which was proposed by our previous work. Digestibility of crude protein, acid ether extract, and nitrogen-free extract varied in a wide range among feedstuffs, even among three samples of feather meal. The digestibility of crude protein, acid ether extract, and nitrogen-free extract as well as the DE content showed a significant negative correlation with crude fiber contents among 13 samples of 12 feedstuffs other than animal by-product feedstuffs and cornstarch. The system proposed by NRC to evaluate dietary metabolizable energy (ME) in dogs tended to underestimate the energy value of some feedstuffs, especially low-fiber starchy feedstuffs such as cereals and cornstarch, but tended to overestimate other feedstuffs, especially high-fiber feedstuffs such as wheat bran and rice bran. This was due to the fixed digestibility of proximate components adopted by NRC. Present results suggest that the MEn obtained by applying actually determined DE and DCP to the above equation would be more reliable than the ME values estimated according to the NRC system.
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  • Masayuki Funaba
    2000 Volume 3 Issue 2 Pages 82-97
    Published: October 01, 2000
    Released on J-STAGE: September 24, 2012
    JOURNAL FREE ACCESS
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  • Atsushi Murai, Mitsuhiro Furuse
    2000 Volume 3 Issue 2 Pages 98-106
    Published: October 01, 2000
    Released on J-STAGE: September 24, 2012
    JOURNAL FREE ACCESS
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  • Yasuki Nonaka
    2000 Volume 3 Issue 2 Pages 107-116
    Published: October 01, 2000
    Released on J-STAGE: September 24, 2012
    JOURNAL FREE ACCESS
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  • Masami Muto, Keiko Horiguchi, Fujiro Sakurai
    2000 Volume 3 Issue 2 Pages 117-123
    Published: October 01, 2000
    Released on J-STAGE: September 24, 2012
    JOURNAL FREE ACCESS
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  • 2000 Volume 3 Issue 2 Pages 124-128
    Published: October 01, 2000
    Released on J-STAGE: September 24, 2012
    JOURNAL FREE ACCESS
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