[in Japanese]

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It was investigated the dose response curve and reproducibility of assay method of the PMSG in dd mice.
The positive rate of hemorrhagic f ollices and corpora lutea forming reaction showed a tendency to increase with increase in dose levels of the PMSG ranging from 4.5 to 10.0 International Unit, but the response of uterine weight showed a trend to increase was observed in a dose of the PMSG ranging from 1.0 to 2.0 International Unit.
There were no significant differences in the results of repeated experiments carried out by the 4 point assay.
Therefore, it was found that the method by means of uterine weight and hemorrhagic follicles and corpora lutea forming reactions had reproducibility in dd mice.

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It was investigated the specific estimation of the HCG and PMSG in mixed gonadotropic preparation by the biological neutralization reaction with each antiserum and hemagglutination inhibition reaction (abb. Bio-Immunological method).
In the cross biological neutralization reaction and hemagglutination inhibition reaction of the HCG and PMSG, a specific reaction was induced the HCG against anti-HCG serum and the PMSG against anti-PMSG serum, but not induced between ES, HS, or NRS.
The Bio-Immunological method was possible to estimate specifically the HCG and PMSG activity in the mixed gonadotropic preparation.

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The Bronze turkey pullet, 10-month-old was inseminated with 0.1ml of undiluted semen obtained from the White Holland tom, 38-month-old. The fertility, hatchability and the duration of fertile eggs following a single insemination was 97.3%, 83.4% and 61 days respectively. This 61 days duration of fertile eggs was fairly above the average. These results will provide with evidence of greater potential in the practical application of artificial insemination of turkeys in the future.

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Factorial experiments were conducted to clarify the effects of several factors on the motility of stallion spermatozoa after pellet freezing and other freezing methods.
1) Concentrated frozen semen in pellet form, after preliminal dilution 1:1 with egg-yolk sugars (glucose, lactose and raffinose)-glycerol (7.5% ) diluent (GLR freezing media), showed better spermatozoan motility after thawing than unconcentrated frozen samples diluted directly 1:1 with GLR freezing media. The results suggest that using of concentrated semen for freezing is not only a mean of economical storage, but also a method of improving the post-thaw motility (Table 1, 2, 3).
2) It was successful to add GLR freezing media to the concentrated semen without decrease of spermatozoan motility at a time at room temperature or 5°C. Motility difference between the pellet and the straw freezing was not significant.
3) In experiment to investigate best semen conditions for centrifugation, there was no difference in post-thaw motility of spermatozoa among samples which were diluted at the ratio from one fourth to equal volume of raw semen with egg-yolk glucose madia as a preliminal diluent.
In cases of centrifugation of raw or yolk diluted semen, the motility were worse than the above conditions but better than uncentrifuged frozen semen.
4) The diluted semen with GLR freezing media was able to cool rapidly to 5 C from room temperature (25°C) by direct setting the semen test tubes into refrigerator (5 C) or plunging them into ice water (Table 4).
Tested volume of diluted semen to be cooled rapidly had no effect on the post-thaw motility of spermatozoa (Table 5).
5) Post-thaw motility was compared between pellet, straw and ampoule (LN vapour) frozen semen, . and several final glycerol levels in diluted semen.
There was no significant difference between pellet and straw, or straw and ampoule freezing, respectively. But difference between pellet and ampoule freezing was significant.
Concernig on glycerol level, significant difference was only shown between 0% leveled and other leveld samples (Table6).
6) Fron storage experiment of pellet frozen semen, post-thaw motility of stallion spermatozoa was not influenced significantly by stored days, stored temperature and kinds of sugars except glycerol levels, (Table 7 and 8).

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Investigations on the reproductive efficiency and causes of sterility of dairy cattle were performed in an intensive dairy farming area of Tohoku region (north eastern parts of Japan) from June 1964 to August 1965. A total of 3, 520 animals involving 816 mature heifers and 2, 704 cows of Holstein and. Jersey breeds were used as materials. The results obtained were summarized as follows:
1. Five hundred and ten (14.5%) of the animals were diagnosed as non pregnant due to various reproductive disorders, in which ovarian disease (166-32.6%), complication of ovarian and uterine diseases (16832.9%), uterine diseases (11923.3%), repeat breeding (254.9%) and others (316.3%) were included.
2. Silent or feeble estrus, anestrus (quiescent or hypoplastic ovary) and continued estrus (ovulation failure) were main symptoms of the ovarian diseases. The most frequent uterine diseases were catarrhal endometritis. Uterine abnormalities palpable per rectum were also found frequently.
3. In the cow group, cases of silent or feeble estrus, quiescent ovary and complication of quiescent ovary and endometritis occourred frequently. In the heifer group, on the other hand, endometritis, ovarian hypoplasia and repeat breeding were predominant.
4. During the investigation period, 352 haifers and 1, 076 cows became pregnant, the number of the heifer and cow conceived within 3 services was 317 (90.1%) and 960 (89.2%) respectively. The average number of service per conception was 1.98 and 1.96 respectively.
5. The interval from parturition to the next conception seemed to be longer in the cow calved from April to June and from November to December than in those calved in the other months.
6. It was considerd that most of the reproductive disorders were caused by poor management at raising term in the heifer and at prepartum to early lactation period in the cow.

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[in Japanese]

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