[in Japanese]

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Immunological cross-reactivity of anterior pituitary gonadotrophin of equine, ovine, porcine, lapine, chicken and rat origins, pregnant mare serum gonadotrophin (PMSG) and human chorionic gonadotrophin (HCG) with antiserum to bovine luteinizing hormone (LH) was studied by the methods of agar gel double diffusion and hemagglutination inhibition reaction.
In agar gel double diffusion, saline extracts of anterior pituitary of bovine, ovine, porcine, lapine and rat origins developed precipitin line against antiserum to bovine LH absorbed with bovine serum, while extracts of equine and chicken origins did not.
Anterior pituitary total gonadotrophin (APG) preparations of bovine, ovine and porcine origins also, developed precipitin line against the antiserum, but the preparation of equine origin did not. These results were in good agreement with that of the saline extracts of anterior pituitary.
Purified LH preparations of bovine and ovine origins developed respectively a single precipitin line against the antiserum to bovine LH absorbed with bovine serum and thyroid stimulating hormone (TSH). As they fused completely each other at the terminal, it seems that the antigenicities of bovine and ovine LH are identical.
None of precipitin line appeared when PMSG and HCG reacted with antiserum to bovine LH.
In hemagglutination inhibition reaction, APG preparations of bovine, ovine and porcine origins and purified LH preparations of bovine and ovine origins inhibited the hemagglutination reaction of bovine LH sensitised cell and the antiserum to bovine LH, while APG preparation of equine origin, PMSG and HCG did not.
These results were in good agreement with that of agar gel double diffusion.
It seems that the antiserum to bovine LH is applicable for the immunoassay of APG as LH of ovine, porcine, lapine and rat origin, but not for APG of ovine, porcine, lapine and rat origin, but not for APG of equine and chicken origin, and PMSG and HCG.

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Immunological cross-reactivity of anterior pituitary gonadotrophin of bovine, equine, ovine and porcine origin and pregnant mare serume gonadotrophin (PMSG) with antiserum to human chorionic gonadotrophin (HCG) was studied by the methods of agar gel double diffusion and hemagglutination inhibition reaction.
Hemagglutination inhibition reaction was examined by a reagent of commercial pregnancy test.
None of the anterior pituitary total gonadotrophin preparations of bovine, equine, ovine and porcine origins and PMSG developed precipitin line against antiserum to HCG absorbed with urine of child in agar gel double diffusion plate.
Purified gonadotrophins, luteinizing hormone (LH) of bovine, equine and ovine origins and follicle stimulating hormone (FSH) of ovine porcine origins, also developed no precipitin lines against antiserum to HCG absorbed with urine of child in agar gel double diffusion plate.
All of these gonadotrophic hormone preparations did not inhibit the hemagglutination reaction of HCG sensitized blood cells and antiserum to HCG.
These results indicate nonexistence of cross-reactivity between gonadotrophins of various species of animals and antiserum to HCG.
It seems, therefore, antiserum to HCG in not applicable for the immunoassay of animal gonadotrophins.

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The differentiation of blastomers to inner cells and trophoblasts in the hamster is observed in all of the 8-celled eggs 7076 hours after ovulation. The differentiated 8-cell egg consist of an inner 4-cell mass and four trophoblasts. It is found that a lecithocoele appears already in this stage. All, differentiated 16-cell eggs (7477 hours after ovulation) also have an inner 8-cell mass, eight trophoblasts and the lecithocole. Developing the differentiated 8-cell eggs to differentiated 16-cell eggs, the differentiated 16-cell eggs themselves shrink and become flat. And so the perivitelline space is seemed to be enlarged. In the blastocyst stage, the eggs become again larger in size, accompanying the enlargement of ecithocoele, but the number of composing cells do not change.

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Histochemical demonstration of dehydrobenases, nonspecific esterase and phosphatases have done in the ovarian interstitial tissue of rabbits, hamsters and mice. The results obtained were summarized as follows.
While there was always Δ⁵-3β-hydroxysteroid dehydrogenase in the interstitial tissue of these rodents, there was no detectable 3α- and 17β-hydroxysteroid dehydrogenases. Glucose-6-phosphate dehydrogenase which was closely related to steroid dehydrogenase was observed in the interstitial tissue. Also isocitrate, malate, succinate and a-glycerophosphate dehydrogenases were demonstrated, but lactate, β-hydroxybutyrate, glutamate dehydrogenases were scarcely demonstrated. The interstitial tissues had nonspecific esterase and acid phosphatase, whereas no alkaline phosphatase was observed.
Significant differences were found between the primary and secondary interstitial tissues of hamsters in the reaction of Δ⁵-3β-hydroxysteroid hydrogenase, glucose-6-phosphate dehydrogenase, nonspecific esterase, alkaline and acid phosphatases. So far as hamster was concerned, no change of enzyme activities was observed during estrus, pergnancy and lactation. In rabbits, however, a-glycerophosphate dehydrogenase activity enhanced in pregnancy. In mature mice that received superovulation treatment, there was no change of enzyme activities in the interstitial tissue.
Change of enzyme activities during estrus, pregnancy and lactation, and those caused by superovulation treatment were compared between interstitial tissues and corpora lutea of these rodents.

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