This experiment has been carried out in order to examine the possibility of use of Euvestin sol (diethylstilbene+diethylstilbestrol) instead of Euvestin oil (diethylstilbene) for the convenience of practical application in the induction of lactation, fy investigating the levels of estrogen in the blood of goats treated with Euvestin sol or oil, and the relation between their levels and lactation results induced by Euvestin injection.
Animals used were five virgin goats, and among them, three were injected intramuscularly with Euvestin sol and the others were subcutaneously with Euvestin oil. Free and protein bound estrogen levels in the blood were measured by the mice intravaginal assay method. Milking was done once a day from the day of the first injection. As the lactation results, total milk yield, average daily yield, maximum period, duration of lactation and average butter fat percentage were measured.
The results obtained are summarized as follows:
( 1 ) Pretty remarkable differences were shown among free and protein bound estrogen levels in the blood at the time of the first injection. Thereafter, free estrogen levels increased and maintained in comparatively high levels about from 12th to 15th day and afterwards decreased, excepting D. Concerning to protein bound estrogen, no definite tendency was seen. Both free and protein bound estrogen levels on the 23rd day were lower than those at the time of first injection.
( 2 ) In either Euvestin sol or oil group, no relation was found between results of induced lactation and estrogen levels in the blood.
( 3 ) There was no difference in total milk yield and other characters, owing to the conditions and injection methods of Euvestin.
( 4 ) From the results of this experiment, the use of Euvestin sol for induced lactation seems to be plausible.

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The increase in the R-fraction (total base determined by resin method, mEq/l-chlorine mEq/l) in the plasma of hen during egg-laying period was reported in the previous paper. Another type of investig-ation of this phenomenon was performed using the serum of estrogenized rooster. The amounts of diff-usible and non-diffusible calcium were determined by an ultrafiltration technique. The results confirm that the lipoprotein in the serum of estrogenized rooster is responsible for the increases in the R-fraction and in the protein-bound calcium.

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[in Japanese]

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Low-density lipoprotein (LDL) was separated from the blood serum of the hen by an ultracentrifugal floatation method. Phosphoprotein (LV) was sedimented to the bottom fraction together with other serum proteins of high density. Isolated LDL was soluble in water over a pH range of 9-4, however, rendered insoluble when mixed with LV at an acid pH. Heparin or dextran sulfate also formed insoluble complexes with LDL, and hence the amount of LDL in serum could be determined by measuring the produced turbidity. The titer of serological vitellin reaction of LDL was several times as high as that of LV.
It was assumed that most of yolk lipids might have their origin in LDL of the serum.

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[in Japanese]

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