Antigonadotrophin formation was produced by the repeated injection of 5 i. u. per days with pregnant mare's serum gonadotrophin and chorionic gonadotrophin into immature female mice.
These antigonadotrophin were recognized after thirty days in the tests of P. M. S. G. injection groups, and after sixty days in the C. G. injection groups.
This antigonadotrophic activity seems to be augmentative as these substances were injected continuously.
Upon the ninety days examination, it was unchanged upon the ovarian weights between injected groups and controls.
It is hard to explain how antigonadotrophin formation cause the increasing of basophiles percentages in hypophysis, however, upon this experiment the increases of these cell were demonstrable in the most cases.

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Althongh a considerable number of studies on the antihormone against human chorionic gonadotrophin (HCG) administered in the man and laboratory animals such as the rat and rabbit have reported, a little is known about domestic animals.
We performed investigations on the antihomone against HCG (Anti-HCG) in cattle receiving this hormone. Gonadotrophic potencies of HCG used 'in peresent study were assayed biologically and the results obtained are shown in Fig. 1.
In this paper an attempt was made to ascertion the Anti-HCG formation in the sera of a female calf (No. 1 : 3 months of age) and a cow (No. 2 : 8 years of age). The 2 animals were injected intravenously with large quantities of HCG tolerably purified. Doses of HCG injected, intervals of the injection and times of blood collections from them were as shown in figures presented under Tables 2 and 3. Every sample was determined whether it posessed Anti-HCG, by emloying immature female mice, as shown in Table 1.
Results of the determinations of Anti-HCG in the serum samples from the 2 animals were shown in Table 2 and Table 3 respectively. The results obtained were summarized as follows:
1. In cattle No. 1 which was injected with 29, 230 MU of HCG (equivalent nearly to 5 MU per cc. of the blood), the determination of Anti-HCG was negative on the serum collected at 19 days after the HCG injection. Then the animal was received 20 injections of 1, 000 MU of HCG for 39 days. On the 11 th day after the last injection, Anti-HCG was determined as positive, the inhibiting titers of the serum being 6 MU per cc.
2. In cattle No. 2 which was injected with 137, 000 MU of HCG (same blood level as in No. 1), the determination of Anti-HCG was positive on the serum of 17 days after the injection.
3. The serum samples collected from the 2 animals on the 50 th day after the HCG injection revealed inhibitory activities against HCG, the inhibiting titer being about 1, 5-3, 0 MU per cc. Then the determinations were negative on the 100 th day.
4. It was clearly demonstrated that the antihormone was produced in cattle injected with large quantities of HCG.

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In peevious report we observed that the antihormone against HCG was produced in cattle receiving large amounts of this hormone. The dosage of HCG used in the previous experiment, however, was much more than that used generally for the purpose of treating ovarian disorders in cattle. In this paper an attempt was undertaken to ascertain whether the Anti-HCG was produced in cattle which had been injected repeatedly with general dosage of HCG. Serum samples collected from 11 cows with cystic ovaries were used as materials to determine the Anti-HCG. The methods to determine the Anti-HCG in the serum samples were same as described in the previous report. These animals had been injected 13 times with 5, 000 IU-20, 000 MU of HCG for treating ovarian cysts. The treating histories, prognosis and ovarian conditions of these animals and the results of the Anti-HCG determinations are shown in Table 1. The Anti-HCG determinations were also performed on the serum samples collected from 11 control animals which had not been injected with HCG within recent 12 months. The ovarian conditions and the results of the Anti-HCG determinations on the control animals are shown in Table 2.
The results obtained were smumarized as follows:
1. In 6 of 11 cows which had been received 13 injections of 5, 00020, 000 MU or 5, 00010, 000 IU of HCG intramuscularly within recent 4 months in order to treat ovarian cysts, the serum Anti-HCG was determined to be positive. The HCG inhibiting titers of these positive sera were 1.5 MU-24.0 MU or more per cc. The Anti-HCG positive animals did not recover from ovarian cysts in spite of the HCG treatments for 13 times.
On the contrary, in 15 animals in which the serum Anti-HCG was determined to be negative, 2 animals had recovered from ovarian cysts and came in normal estrous cycle and 2 were recovering from ovarian cysts by the HCG treatments. Only one animal was not yet restored by the treatments.
From these results, it is possible that the Anti-HCG in produced in considerable number of cattle with cystic ovaries which were uncurable following repeated treatments with HCG. 2. On all of the serum samples from 11 control animals, including 5 with cystic ovaries, 3 with ovarian dysfunctions and 3 with normal estrous cycles, the Anti-HCG was determined to be negative.
From these results, it is quite possible that the Anti-HCG is not produced in cattle which have not been injected with HCG.

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In the fowl, the relationship between the dilution rate and the fertility of semen have beer reported by some workers but there is very little information available in the duck.
In the present experiments, semen was collected electrically; a composite sample from five 8-month-old drakes was diluted 5 times with the fowl egg-yolk citrate buffer, gradually cooled to 5°C withir a half hour and stored in a refrigerator at 5°C. Thirty ducks of 8-months of age were divided into flocks, each consisting of 6 ducks ( Flock I to V). Each diluted semen sample was injected simultaneously to each flock with a dose of 0.3 ml. per bird. Fertility tests were made with the semen samples stored for 0, 24, 48, 72 and 96 hours respectively.
The results of the last two gronps were quite negative, no fertile egg being produced ; thence the results obtained in Flock IV and V were omitted from Table 1. Results are summarized as follows:
1. In this medium the semen injected immediately after collection showed 100 percent daily fertility with a slight intermediate decreasing of the rate from the third to fifth day following insemi-nation. The fertility potential was maintained until the twelfth day after insemination (Table 1, Flock I).
2. The Semen of 24-hour storage showed 83.3 percent fertility on the third day following insemination; the fertility potential was maintained until the nineth day (Table 1, Flock II).
3. The daily fertility of the semen stored for 48 hours was almost the same as that of the semen stored for 24 hours. However, the duration retaining the fertility potential was quite different; it was maintained only for 5 days in the case of 48-hour storage (Table 1, Flock III).

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