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Volume 16 (1997)
- Issue 4 Pages 181-
- Issue 3 Pages 147-
- Issue 2 Pages 111-
- Issue 1 Pages 14-
Volume 16, Issue 4
Displaying 1-5 of 5 articles from this issue
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[in Japanese], [in Japanese]1997 Volume 16 Issue 4 Pages 181-187
Published: December 31, 1997
Released on J-STAGE: November 13, 2012
JOURNAL FREE ACCESS -
Miho FURUE, Shigeru SAITO1997 Volume 16 Issue 4 Pages 189-194
Published: December 31, 1997
Released on J-STAGE: November 13, 2012
JOURNAL FREE ACCESSTo examine the effect of hepatocyte growth factor (HGF) and fibroblast growth factor-1(FGF-1) on the regeneration of submandibular gland (SMG), rat SMG (RSMG) epithelial cells were primary cultured in serum-free medium. FGF-1 and HGF individually promoted the proliferation of RSMG cells in a dose-dependent manner, and FGF-1 remarkably promoted the proliferation effect of HGF. In three-dimensional collagen gel culture, RSMG cells were able to form branching structures. HGF potently induced branching morphogenesis of RSMG cell colonies embedded in collagen gel. RSMG cells treated with FGF-1 grew into a star-like colonies, but not further. However, FGF-1 remarkably promotes the morphogenic effect of HGF, assuring that the colony cultured in the presence of both FGF-1 and HGF formed more branches than colonies growing in the presence of only one growth factor. Paraffin section of the RSMG cells embedded in collagen gel revealed well-formed lumina in the presence of HGF and FGF-1. This morphology resembles closely the one found in situ. These findings suggest that HGF induces the proliferation and branching morphogenesis, and FGF-1 enhances the effect of HGF in the SMG regeneration. hepatocyte growth factor, fibroblast growth factor, salivary gland, morphogenesis, regenerationView full abstractDownload PDF (2387K) -
Tadanori TANAHASHI, Takehiro ITOH, Masashi SUZUKI, Toru IMAMURA1997 Volume 16 Issue 4 Pages 195-199
Published: December 31, 1997
Released on J-STAGE: November 13, 2012
JOURNAL FREE ACCESSWe have previously demonstrated that only when serum was included in the culture medium, heparin-binding fibroblast growth factor-1 (FGF-1) was a potent mitogen for rat hepatocytes in primary culture. Here we have defined the constituents of serum that is important for the mitogenic activity of FGF-1. Addition of dialyzed serum to the basal medium supported about half of the FGF-1 activity compaired to whole fetal bovine serum (FBS). Further addition of ascorbic acid 2-phosphate to this medium reconstituted the full activity of FGF-1. However, ascorbic acid 2-phosphate alone could only partially support the FGF-1 mitogenicity. These findings indicate that dialyzed-FBS and ascorbic acid 2-phosphate additively or synergistically support the mitogenic activity of FGF-1. These effects of dialyzed FBS and ascorbic acid 2-phosphate were observed regardless of the basal medium used, i. e., Williams'medium E or MCDB 107 medium. Thus, these results suggest that the high molecular-weight factor(s) and ascorbic acid-like compound(s) are the important constituents of serum that support the FGF-1 mitogenic activity.View full abstractDownload PDF (1336K) -
Mana MATSUMOTO, Natsuki MATSUMOTO, Yukihiko HARA, Yukiaki KURODA1997 Volume 16 Issue 4 Pages 201-205
Published: December 31, 1997
Released on J-STAGE: November 13, 2012
JOURNAL FREE ACCESSHuman diploid cell strains had tissue- or organ-specific lifespans depending on differences in the sources of cells. In most of the previous studies on the lifespan of embryonic chick cells, mixtures of different tissues and organs were used as the source of cells. In the present study, the lifespans of femur chondrocytes and cardiac muscle cells obtained from the same 13-day chick embryos were compared under identical serial cultivations. Chondrocytes divided more rapidly than cardiac muscle cells. The average increasing rates of chondrocytes per day stayed at levels of more than 1.0 for first 8 passages. On the other hand, those of cardiac muscle cells were at levels of more or less than 0.5 for 11 passages. The cell cycle time of the former was about half that of the latter. The average cell cycle times of chondrocytes were 27-51 h in the first 7 passages, whereas those of cardiac muscle cells were 53-97 h in the first 5 passages. The number of population doublings (PDs) of chondrocytes was 29.6 with 19 passages in cultivation for 98 days, whereas PDs of cardiac muscle cells was 13.3 with 13 passages for 85 days. This may be the first report to compare the lifespans of chondrocytes and cardiac muscle cells of the embryonic chick.View full abstractDownload PDF (1184K) -
Yoshiharu Tanaka, Akihiko Sakamoto, Zhang Yan, Eiji Michimukai, Shigea ...1997 Volume 16 Issue 4 Pages 207-213
Published: December 31, 1997
Released on J-STAGE: November 13, 2012
JOURNAL FREE ACCESSMalignant salivary gland tumors are devasting neoplasm that is associated with a poor prognosis in the head and neck regions. Elevated expression of fibroblast growth factors (FGFs)in malignant salivary gland tumors has implicated the FGF family of mitogens in the initiation and progression of salivary gland-derived tumors. In this study, we demonstrated that human salivary gland tumor undergo parallel changes in FGF receptor (FGFR) expression during their progression from a benign to a malignant phenotype. An FGFR2-(IIIb) expression was abundant in normal human salivary gland-derived epithelial (HSGE) cells and in all benign salivary gland tumors but was not seen in malignant salivary adenocarcinoma cells. On the other hand, FGFR1 and FGFR4 expression was absent in HSGE cells and in all benign salivary gland tumors but was significantly elevated in malignant salivary gland adenocarcinoma cells. These results suggest that differential expression and alternative splicing of FGFRs may be critical in the malignant progression of salivary gland tumors.View full abstractDownload PDF (2044K)
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