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Volume 26 (2007)
- Issue 4 Pages 171-
- Issue 3 Pages 149-
- Issue 2 Pages 115-
- Issue 1 Pages 21-
Volume 26, Issue 3
Displaying 1-3 of 3 articles from this issue
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Kiyoshi AKEO, Tomoo FUNAYAMA, Nobuyuki HAMADA, Yoko AKEO, Tadahisa HIR ...2007 Volume 26 Issue 3 Pages 149-157
Published: 2007
Released on J-STAGE: November 30, 2007
JOURNAL FREE ACCESSGlutathione peroxidase (GPX) protects the phospholipids in the biomembrane barrier from damage by the free radicals. We studied cultured bovine retinal pigmented epithelial (RPE) cells exposed to UV-A and UV-B to try to determine the expression of GPX and glyceraldehdye-3-phosphate dehydrogenase (GAPDH) that are highly sensitive to oxidation. The total cellular RNA of the RPE cells was reverse transcribed by using primers for GPX and GAPDH. The quantitative real-time RT-PCR was performed to compare the effects of UV-A and UV-B irradiation on 18S ribosomal RNA (rRNA) and GPX expression using the LightCycler system. The expression of GAPDH and GPX was decreased by hyperoxia such as 20% oxygen and UV-B irradiation using electrophoresis. The LightCycler analysis, the quantitative real-time RT-PCR, showed that UV-A exposure at a small dose induced the defense mechanism against lipid peroxidation in RPE cells. However, UV-A exposure at a large dose led to a disturbance in the defense mechanism similar to that observed with UV-B irradiation; this could have a relationship with retinal diseases caused by light damage.View full abstractDownload PDF (1072K) -
Arihiro Kohara, Azusa Ohtani, Yutaka Ozawa, Setsuko Shioda, Tohru Masu ...2007 Volume 26 Issue 3 Pages 159-163
Published: 2007
Released on J-STAGE: November 30, 2007
JOURNAL FREE ACCESSMycoplasmas are smallest self proliferating microorganism of about 1/10 of a bacterium. While they are existing with cell cultures, it is difficult to realize because medium does not become turbid. But we should not ignore the mycoplasma contaminations because they induce many bad effects for our researches using cell cultures. For example they induce cell death, abnormal induction of cytokines, or chromosome aberrations. Thus, the Japan Tissue Culture Association recently started a nationwide investigation for the mycoplasma contaminations with cooperatively with the JCRB cell bank by using the inspection kit called “MycoAlert®”. We have analyzed about 1500 samples already, and average rate of the contamination was 22.4%. We would like to identify contaminating mycoplasma species, and to specify the infection route in future. We also would like to introduce decontamination methods.View full abstractDownload PDF (640K)
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Hiroshi Mizusawa, Tohru Masui, Masao Takeuchi, Arihiro Kohara2007 Volume 26 Issue 3 Pages 165-170
Published: 2007
Released on J-STAGE: November 30, 2007
JOURNAL FREE ACCESSWe usually store the cultured cells in the liquid nitrogen system. A vapor phase system with -190°C large container is good to prevent mycoplasma contaminations and for the long period safe storage. We obtained such a large container cell storage system with -190°C recently, whose inner materials were changed to aluminum. Although the system works nicely but we realized that it should be handled quickly when getting out ampoules because of the quick temperature change.View full abstractDownload PDF (1034K)
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