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Volume 29 (2010)
- Issue 2+3+4 Pages 147･･･
- Issue 1 Pages 27-
Volume 29, Issue 2+3+4
Displaying 1-3 of 3 articles from this issue
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Ran GAO, Tomoko YAGUCHI, Katsuhisa HORIMOTO, Tetsuro ISHII, Sunil C. K ...2010 Volume 29 Issue 2+3+4 Pages 147-153
Published: 2010
Released on J-STAGE: January 27, 2011
JOURNAL FREE ACCESSMortalin is a member of the Hsp70 family of proteins and exhibits pancytoplasmic distribution pattern in normal and perinuclear in cancer cells. Human cancer cells when induced to senesce by a variety of chemicals and stress conditions show shift in mortalin staining pattern from perinuclear to pancytoplasmic type along with the nuclear translocation and activation of p53 function. Using mortalin staining as a model reporter, we screened human shRNA library for candidates with anticancer activity. Cancer cells were stably transfected with the shRNA expressing plasmids in 96-well plates, immunostained with anti-mortalin antibody and screened for mortalin staining pattern. By four rounds of screening, we have identified nine shRNAs that caused shift in mortalin staining from the perinuclear (cancer cell type) to the pancytoplasmic (normal cell type) pattern. Furthermore, the effect of selected shRNAs was studied on p53 and p16 pathways. Bioinformatics approach revealed the involvement of DNA damage signaling pathway along with NBS1, MRE11 and BRCA1 as candidate targets for anticancer therapy.View full abstractDownload PDF (571K) -
Nana Ninagawa, Rumi Murakami, Yusuke Tanaka, Hiroki Nakagawa, Shigeko ...2010 Volume 29 Issue 2+3+4 Pages 155-165
Published: 2010
Released on J-STAGE: January 27, 2011
JOURNAL FREE ACCESSMesenchymal stem cells (MSCs) have the potential to differentiate into mesenchymal cells. MSCs have been collected from several adult tissues including bone marrow and adipose tissues. They are named bone marrow-derived stem cells (BMSCs) and adipose tissue-derived stem cells (ADSCs), respectively. Isolation and collection of BMSCs and ADSCs, however, remains limited in number, and requires invasive and troublesome procedures. Furthermore, MSCs derived from adult tissue have a limited life span. Thus, there are some difficulties in obtaining a large number of MSCs from adult tissue. Mouse embryonic stem cells (M-ESCs) are pluripotent. Here we report a novel method for induction and isolation of ADSCs with high probability and purity from M-ESCs through adipogenesis without gene manipulation. After 8 days of adipogenesis from M-ESCs, we found ADSC-like cells expressing cell surface antigen CD105. Subsequently, CD105+ cells were collected by magnetic cell sorting (MACS). The high purity of isolation by MACS was confirmed by flow cytometry. Obtained CD105+ cells revealed a potential to differentiate into mesenchymal cells such as adipocytes, osteogenic cells, chondrocytes and skeletal muscles in vitro. The results indicate that sorted CD105+ cells correspond to ADSCs and suggest that M-ESCs are a new source of ADSCs.View full abstractDownload PDF (1278K) -
Aya KASAMATSU, Motonobu SATOH, Touho YOSHIDA, Takuo KOSAKA2010 Volume 29 Issue 2+3+4 Pages 167-172
Published: 2010
Released on J-STAGE: January 27, 2011
JOURNAL FREE ACCESSThe Health Science Research Resources Bank (HSRRB), the first public human tissue bank in Japan, has been providing human cells prepared from remnant tissues obtained from surgeries performed on Japanese patients to academia and industry under the approval of institutional review board (IRB). HSRRB possesses several lots of fibroblast-like synoviocytes (FLSs) derived from synovial tissues of Japanese patients with rheumatoid arthritis (RA). FLSs are closely related to the pathogenesis of RA. Here we report the quality controls on FLSs revealing the features reflecting RA. We measured the production of matrix metalloproteinase (MMP) -3 and interleukin (IL) -6 in the cells following tumor necrosis factor-alpha (TNF-α) treatment at protein and mRNA levels using ELISA, Western blotting, and RT-PCR methods. As a result, we found that all lots of FLSs maintained a significant inflammatory response to TNF-α during several passages, suggesting a physiological aspect in RA.View full abstractDownload PDF (674K)
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