For the purpose of clarifying the reaction of the sutursite stimulated by standardized and bodily expansion, the following
in vitroexperiments were performed using the parietal bones of the newborn Polish-Dutch rabbit.
Preliminary experiment: To learn about the site, length and conditions for the culture
Main experiment: 1. To develop a culture apparatus to expand the suture to the standardized width
2. To observe the tissue reaction after the expansion when applying this apparatus
Additional experiment: To examine a tetracycline labeling
The results were as follows:
1) It was necessary to maintain the width of the suture by some method for culturing the suture.
2) Under conditions where O
2is 50%, it is appropriate to have at least 5 days for the culture period where the enzyme in the medium consists of horse serum and chick embryo extract.
3) An orthodontic rapid expansion screw was applied to the apparatus to give a standardized displacement to the suture, called the hanging technique.
4) The tension group, which expanded 200 μ in width with this apparatus, indicated the following on the 4 th-days.
1. Increase of fibroblasts
2. Tension of the fibres
3. Extensive appearance of an immature bone-like tissue
4. Possible calcification judging from the findings of tetracycline labeling and CMR method
5) It was strongly suggested that this experimental model could be applied to tooth movement, bone growth and calcification.
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