Eighteen serovars (19 strains) of serogroup Icterohaemorrhagiae were serologically analyzed using 18 monoclonal antibodies against serovar
copenhageni Shiromizu, M20 and serovar
icterohaemorrhagiae RGA strains. The reaction patterns of the serovars against these monoclonal antibodies were different. According to these results, we divided the serovars, except for serovar
tonkini, into the following three subgroups:
Subgroup 1 reacted to many monoclonal antibodies including serovars
icterohaemorrhagiae,
copenhageni, hualien, monymusk, mankarso, and
budapest.
Subgroup 2 fell between subgroups 1 and 3 including serovars
dakota, naam, bogvere,
birkini, smithi, ndambari, gem, ndahambukuje and
mwogolo.
Subgroup 3 reacted to only a few monoclonal antibodies: serovars
weaveri and
sarmin.
Serovar
tonkini did not react to any of the monoclonal antibodies used. There is a possibility that serovar
tonkini does not belong to serogroup Icterohaemorrhagiae. Further studies on the serological reactions of each strain revealed that it was impossible to distinguish the RGA strain from the serovar
hualien LT11-31 strain, indicating that they may be identical. It was also observed that serovar
copenhageni and
monymusk seemed to be closely related. Serovars
birkini and
smithi, and serovars
ndambari and
gem were alike in their serological reactivities.
Among the 18 monoclonal antibodies, RGAMA-1 was a unique antibody which reacted only to serovar
icterohaemorrhagiae and serovar
hualien, indicating that it must be the serovar
icterohaemorrhagiae specific antibody. On the other hand, SHIRMA-2, 5, 6 reacted to all the serovars except for serovars
weaveri, sarmin, and
tonkini. These antibodies exhibited a broad reaction spectrum.
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